Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Highly Selective Fermentation of Waste-Activated Sludge by Alginate-Degrading Consortia
https://orcid.org/0000-0003-0658-4775
https://orcid.org/0000-0003-3060-588X
https://orcid.org/0000-0001-8892-7182
Produced waste-activated sludge (WAS) can be recovered as a promising carbon source in wastewater treatment plants. However, the cell integrity is always ignored, which results in the unwanted release of N and P. In this work, an alginate-degrading microbial consortium (ADC) with a higher percentage of genus Bacteroides (>90%) was enriched. The role of enriched ADC on the productions of methane and volatile fatty acids (VFAs) and cell integrity (via lactate dehydrogenase (LDH) activity and DNA concentrations) was then investigated in WAS fermentation. The results showed that dosing with ADC increased methane production by 53% without P release. Methane production of 440 mL from extracted extracellular polymeric substances was comparable to that from WAS (445 mL). After inhibiting methanogenesis, VFA concentrations via dosing with ADC were comparable to those of alkali fermentation, while expectantly showing lower N (114 vs 367 mg/L) and P (0 vs 122 mg/L) release. Since N and P are mainly stored within cells, such better selectivity was attributed to the ADC not affecting cell integrity, which was also verified by LDH activity and DNA concentrations. Consequently, these results provide a highly selective microbial method to produce biochemicals from WAS while maintaining cell integrity.
Highly Selective Fermentation of Waste-Activated Sludge by Alginate-Degrading Consortia
https://orcid.org/0000-0003-0658-4775
https://orcid.org/0000-0003-3060-588X
https://orcid.org/0000-0001-8892-7182
Produced waste-activated sludge (WAS) can be recovered as a promising carbon source in wastewater treatment plants. However, the cell integrity is always ignored, which results in the unwanted release of N and P. In this work, an alginate-degrading microbial consortium (ADC) with a higher percentage of genus Bacteroides (>90%) was enriched. The role of enriched ADC on the productions of methane and volatile fatty acids (VFAs) and cell integrity (via lactate dehydrogenase (LDH) activity and DNA concentrations) was then investigated in WAS fermentation. The results showed that dosing with ADC increased methane production by 53% without P release. Methane production of 440 mL from extracted extracellular polymeric substances was comparable to that from WAS (445 mL). After inhibiting methanogenesis, VFA concentrations via dosing with ADC were comparable to those of alkali fermentation, while expectantly showing lower N (114 vs 367 mg/L) and P (0 vs 122 mg/L) release. Since N and P are mainly stored within cells, such better selectivity was attributed to the ADC not affecting cell integrity, which was also verified by LDH activity and DNA concentrations. Consequently, these results provide a highly selective microbial method to produce biochemicals from WAS while maintaining cell integrity.
Highly Selective Fermentation of Waste-Activated Sludge by Alginate-Degrading Consortia
https://orcid.org/0000-0003-0658-4775
https://orcid.org/0000-0003-3060-588X
https://orcid.org/0000-0001-8892-7182
Produced waste-activated sludge (WAS) can be recovered as a promising carbon source in wastewater treatment plants. However, the cell integrity is always ignored, which results in the unwanted release of N and P. In this work, an alginate-degrading microbial consortium (ADC) with a higher percentage of genus Bacteroides (>90%) was enriched. The role of enriched ADC on the productions of methane and volatile fatty acids (VFAs) and cell integrity (via lactate dehydrogenase (LDH) activity and DNA concentrations) was then investigated in WAS fermentation. The results showed that dosing with ADC increased methane production by 53% without P release. Methane production of 440 mL from extracted extracellular polymeric substances was comparable to that from WAS (445 mL). After inhibiting methanogenesis, VFA concentrations via dosing with ADC were comparable to those of alkali fermentation, while expectantly showing lower N (114 vs 367 mg/L) and P (0 vs 122 mg/L) release. Since N and P are mainly stored within cells, such better selectivity was attributed to the ADC not affecting cell integrity, which was also verified by LDH activity and DNA concentrations. Consequently, these results provide a highly selective microbial method to produce biochemicals from WAS while maintaining cell integrity.
Highly Selective Fermentation of Waste-Activated Sludge by Alginate-Degrading Consortia
Qian, Ding-Kang (Autor:in) / Geng, Zi-Qian (Autor:in) / Tang, Jie (Autor:in) / Wang, Shuai (Autor:in) / Hu, Zhi-Yi (Autor:in) / Dai, Kun (Autor:in) / van Loosdrecht, Mark C. M. (Autor:in) / Zeng, Raymond Jianxiong (Autor:in) / Zhang, Fang (Autor:in)
ACS ES&T Engineering ; 1 ; 1606-1617
12.11.2021
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
Effects of Base Types on Waste Activated Sludge Alkaline Fermentation and Dewaterability
| British Library Online Contents | 2013