Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Analysis of Unamplified 16S rRNA of Ammonia-Oxidizing Bacteria in Activated Sludge by Spectrophotometry Using Gold Nanoprobes
https://orcid.org/0000-0002-5222-9689
The identification of the bacteria contributing to the water purification processes in these systems is crucial for the development and optimization of wastewater treatment systems by engineers. We developed a simple assay for the colorimetric quantification of bacterial 16S rRNA of functional bacteria extracted from wastewater samples without reverse transcription of RNA or quantitative polymerase chain reaction (qPCR). The heat treatment of the test sample significantly reduced the reaction time (<3 min) and improved the assay sensitivity. Following assay optimization, a calibration curve for 16S rRNA of ammonia-oxidizing bacteria (AOB) of the β subdivision of the class Proteobacteria was created using 16S rRNA extracted from activated sludge samples. The curve was linear above 1.9 × 106 copies/mL of 16S rRNA of AOB, which was 2 orders of magnitude lower than that in our previous studies. Finally, we determined the abundance of 16S rRNA of AOB in activated sludge samples using the calibration curve and compared the results obtained by reverse transcription-qPCR. There was some correlation in the RNA concentrations of AOB determined by both methods. Thus, we have successfully developed an innovative tool to quantify the 16S rRNA concentration of AOB in wastewater treatment processes.
Bacterial 16S rRNA concentration was detected colorimetrically using gold nanoparticles conjugated with DNA probe specific to target bacteria without PCR.
Analysis of Unamplified 16S rRNA of Ammonia-Oxidizing Bacteria in Activated Sludge by Spectrophotometry Using Gold Nanoprobes
https://orcid.org/0000-0002-5222-9689
The identification of the bacteria contributing to the water purification processes in these systems is crucial for the development and optimization of wastewater treatment systems by engineers. We developed a simple assay for the colorimetric quantification of bacterial 16S rRNA of functional bacteria extracted from wastewater samples without reverse transcription of RNA or quantitative polymerase chain reaction (qPCR). The heat treatment of the test sample significantly reduced the reaction time (<3 min) and improved the assay sensitivity. Following assay optimization, a calibration curve for 16S rRNA of ammonia-oxidizing bacteria (AOB) of the β subdivision of the class Proteobacteria was created using 16S rRNA extracted from activated sludge samples. The curve was linear above 1.9 × 106 copies/mL of 16S rRNA of AOB, which was 2 orders of magnitude lower than that in our previous studies. Finally, we determined the abundance of 16S rRNA of AOB in activated sludge samples using the calibration curve and compared the results obtained by reverse transcription-qPCR. There was some correlation in the RNA concentrations of AOB determined by both methods. Thus, we have successfully developed an innovative tool to quantify the 16S rRNA concentration of AOB in wastewater treatment processes.
Bacterial 16S rRNA concentration was detected colorimetrically using gold nanoparticles conjugated with DNA probe specific to target bacteria without PCR.
Analysis of Unamplified 16S rRNA of Ammonia-Oxidizing Bacteria in Activated Sludge by Spectrophotometry Using Gold Nanoprobes
https://orcid.org/0000-0002-5222-9689
The identification of the bacteria contributing to the water purification processes in these systems is crucial for the development and optimization of wastewater treatment systems by engineers. We developed a simple assay for the colorimetric quantification of bacterial 16S rRNA of functional bacteria extracted from wastewater samples without reverse transcription of RNA or quantitative polymerase chain reaction (qPCR). The heat treatment of the test sample significantly reduced the reaction time (<3 min) and improved the assay sensitivity. Following assay optimization, a calibration curve for 16S rRNA of ammonia-oxidizing bacteria (AOB) of the β subdivision of the class Proteobacteria was created using 16S rRNA extracted from activated sludge samples. The curve was linear above 1.9 × 106 copies/mL of 16S rRNA of AOB, which was 2 orders of magnitude lower than that in our previous studies. Finally, we determined the abundance of 16S rRNA of AOB in activated sludge samples using the calibration curve and compared the results obtained by reverse transcription-qPCR. There was some correlation in the RNA concentrations of AOB determined by both methods. Thus, we have successfully developed an innovative tool to quantify the 16S rRNA concentration of AOB in wastewater treatment processes.
Bacterial 16S rRNA concentration was detected colorimetrically using gold nanoparticles conjugated with DNA probe specific to target bacteria without PCR.
Analysis of Unamplified 16S rRNA of Ammonia-Oxidizing Bacteria in Activated Sludge by Spectrophotometry Using Gold Nanoprobes
Nakajima, Meri (Autor:in) / Hirano, Reiko (Autor:in) / Nakaya, Yuki (Autor:in) / Satoh, Hisashi (Autor:in)
ACS ES&T Water ; 3 ; 3113-3120
08.09.2023
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
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