Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Preliminary Study of Geophysical Monitoring of Bioclogging Caused by Bacterial Biopolymer Accumulation in Sands
In situ bacterial colonization and accumulation of biofilms (or biopolymers) in subsurface have a profound effect on the physical and chemical properties of soils, influencing fluid flow and transport properties. Thus, detection of microbially active regions and monitoring of in situ microbial biofilm growth are important for successful application of the microbial soil improvement and remediation. This study examines the feasibility of using geophysical signatures for monitoring the accumulation of biofilms and consequential bioclogging in sands. Column experiments stimulating the production of biopolymers by model bacteria were performed along with the monitoring of changes in responses of P- and S-waves and electrical resistivity. The model bacterium Leuconostoc mesenteroides was selected to produce viscous biopolymer, referred to as dextran, in sucrose-rich media. After 41 days of the column experiment, the permeability decreased more than one order of magnitude as a result of bacterial biopolymer accumulation in sand. There were minimal changes in the P- and S-wave velocities. However, the amplitudes of P- and S-waves showed slight decreases, indicating increases in P- and S-wave attenuation at the measured frequency range, ~10-100 kHz for P-wave and ~1-10 kHz for S-wave. The electrical resistivity increased by ~80-100% as a result of the accumulated biopolymers in pores and an associated decrease in porosity.
Preliminary Study of Geophysical Monitoring of Bioclogging Caused by Bacterial Biopolymer Accumulation in Sands
In situ bacterial colonization and accumulation of biofilms (or biopolymers) in subsurface have a profound effect on the physical and chemical properties of soils, influencing fluid flow and transport properties. Thus, detection of microbially active regions and monitoring of in situ microbial biofilm growth are important for successful application of the microbial soil improvement and remediation. This study examines the feasibility of using geophysical signatures for monitoring the accumulation of biofilms and consequential bioclogging in sands. Column experiments stimulating the production of biopolymers by model bacteria were performed along with the monitoring of changes in responses of P- and S-waves and electrical resistivity. The model bacterium Leuconostoc mesenteroides was selected to produce viscous biopolymer, referred to as dextran, in sucrose-rich media. After 41 days of the column experiment, the permeability decreased more than one order of magnitude as a result of bacterial biopolymer accumulation in sand. There were minimal changes in the P- and S-wave velocities. However, the amplitudes of P- and S-waves showed slight decreases, indicating increases in P- and S-wave attenuation at the measured frequency range, ~10-100 kHz for P-wave and ~1-10 kHz for S-wave. The electrical resistivity increased by ~80-100% as a result of the accumulated biopolymers in pores and an associated decrease in porosity.
Preliminary Study of Geophysical Monitoring of Bioclogging Caused by Bacterial Biopolymer Accumulation in Sands
Ta, Hien Xuan (Autor:in) / Kwon, Tae-Hyuk (Autor:in) / Muhunthan, Balasingam (Autor:in)
Geo-Congress 2014 ; 2014 ; Atlanta, Georgia
Geo-Congress 2014 Technical Papers ; 1654-1663
24.02.2014
Aufsatz (Konferenz)
Elektronische Ressource
Englisch
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