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Control of Suitability of the Culture Daphnia magna Straus for Bioassays of Aquatic Environments, Taking into Account Demographic Indicators of Model Populations
The purpose of this work was to experimentally determine conditional reference demographic indicators of laboratory populations of Daphnia magna Straus (1820). These indicators can be used when determining suitability of the aquaculture for bioassays of aquatic environments. The experiments were carried out with parthenogenetic clones of D. magna, the temperature of the environment was 20 °C, the light period was 12 h. It was found that the maximum lifetime of crustaceans is observed in groups with a density of 25 individuals/dm3. In these groups, other demographic characteristics of D. magna such as the day of the first offspring (10 ± 2 days), average lifetime (79.7 ± 1.0 days), specific fertility (74.3 ± 2.4 days) and other related parameters of well-being were established. Then, it was showed that the culture of D. magna, having the necessary sensitivity to the model toxicant K2Cr2O7, can significantly differ in demographic characteristics from the conventional reference groups. Audit of the conditions of keeping this aquaculture revealed a complex reason for the unsatisfactory state of D. magna: the lack of daily aquaculture care and increased concentration of nitrate ions in the cultivation water. Therefore, regular monitoring of D.magna demographic characteristics can complement procedures of standardization of test-cultures. This will contribute to improving the accuracy of bioassay results.
Control of Suitability of the Culture Daphnia magna Straus for Bioassays of Aquatic Environments, Taking into Account Demographic Indicators of Model Populations
The purpose of this work was to experimentally determine conditional reference demographic indicators of laboratory populations of Daphnia magna Straus (1820). These indicators can be used when determining suitability of the aquaculture for bioassays of aquatic environments. The experiments were carried out with parthenogenetic clones of D. magna, the temperature of the environment was 20 °C, the light period was 12 h. It was found that the maximum lifetime of crustaceans is observed in groups with a density of 25 individuals/dm3. In these groups, other demographic characteristics of D. magna such as the day of the first offspring (10 ± 2 days), average lifetime (79.7 ± 1.0 days), specific fertility (74.3 ± 2.4 days) and other related parameters of well-being were established. Then, it was showed that the culture of D. magna, having the necessary sensitivity to the model toxicant K2Cr2O7, can significantly differ in demographic characteristics from the conventional reference groups. Audit of the conditions of keeping this aquaculture revealed a complex reason for the unsatisfactory state of D. magna: the lack of daily aquaculture care and increased concentration of nitrate ions in the cultivation water. Therefore, regular monitoring of D.magna demographic characteristics can complement procedures of standardization of test-cultures. This will contribute to improving the accuracy of bioassay results.
Control of Suitability of the Culture Daphnia magna Straus for Bioassays of Aquatic Environments, Taking into Account Demographic Indicators of Model Populations
Anna Olkova (Autor:in)
2020
Aufsatz (Zeitschrift)
Elektronische Ressource
Unbekannt
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