Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Microbial Strategies for Cellulase and Xylanase Production through Solid-State Fermentation of Digestate from Biowaste
Solid-state fermentation (SSF) is a promising technology for producing bioproducts from organic wastes. The objective of this study is to assess the feasibility of using digestate as substrate to produce hydrolytic enzymes, mainly cellulase and xylanase, by exploring three different inoculation strategies: (i) SSF with autochthonous microbiota; (ii) non-sterile SSF inoculated with Trichoderma reesei and (iii) sequential batch operation to select a specialized inoculum, testing two different residence times. Native microbial population did not show a significant cellulase production, suggesting the need for a specialized inoculum. The inoculation of Trichoderma reesei did not improve the enzymatic activity. On the other hand, inconsistent operation was achieved during sequential batch reactor in terms of specific oxygen uptake rate, temperature and enzymatic activity profile. Low cellulase and xylanase activities were attained and the main hypotheses are non-appropriate biomass selection and some degree of hydrolysis by non-targeted proteases produced during fermentation.
Microbial Strategies for Cellulase and Xylanase Production through Solid-State Fermentation of Digestate from Biowaste
Solid-state fermentation (SSF) is a promising technology for producing bioproducts from organic wastes. The objective of this study is to assess the feasibility of using digestate as substrate to produce hydrolytic enzymes, mainly cellulase and xylanase, by exploring three different inoculation strategies: (i) SSF with autochthonous microbiota; (ii) non-sterile SSF inoculated with Trichoderma reesei and (iii) sequential batch operation to select a specialized inoculum, testing two different residence times. Native microbial population did not show a significant cellulase production, suggesting the need for a specialized inoculum. The inoculation of Trichoderma reesei did not improve the enzymatic activity. On the other hand, inconsistent operation was achieved during sequential batch reactor in terms of specific oxygen uptake rate, temperature and enzymatic activity profile. Low cellulase and xylanase activities were attained and the main hypotheses are non-appropriate biomass selection and some degree of hydrolysis by non-targeted proteases produced during fermentation.
Microbial Strategies for Cellulase and Xylanase Production through Solid-State Fermentation of Digestate from Biowaste
Laura Mejias (Autor:in) / Alejandra Cerda (Autor:in) / Raquel Barrena (Autor:in) / Teresa Gea (Autor:in) / Antoni Sánchez (Autor:in)
2018
Aufsatz (Zeitschrift)
Elektronische Ressource
Unbekannt
Metadata by DOAJ is licensed under CC BY-SA 1.0
| DOAJ | 2016
Optimization of solid fermentation of cellulase from Trichoderma koningii
| Online Contents | 2004
Composting and Fermentation of Biowaste - Advantages and Disadvantages of Processes
| DOAJ | 2018