Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Differential Regulation of Anthocyanins in Cerasus humilis Fruit Color Revealed by Combined Transcriptome and Metabolome Analysis
Coloring is an important appearance quality of fruit. In order to evaluate the relationship between metabolites and fruit color, we analyzed the metabolites and transcriptional profiles of two different Cerasus humilis cultivars: “RF” (cv. Zhangwu, red fruit) and “YF” (cv. Nongda No.5, yellow fruit). The results of identification and quantification of metabolites showed that there were significant differences in the contents of 11 metabolites between RF and YF. Transcriptomics was used to analyze the expression patterns of genes related to the anthocyanin biosynthesis pathway, and subsequently, the regulation network of anthocyanin biosynthesis was established to explore their relationship with color formation. QRT-PCR, performed for 12 key genes, showed that the expression profiles of the differentially expressed genes were consistent with the results of the transcriptome data. A co-expression analysis revealed that the late genes were significantly positively correlated with most of the different metabolites. The results of the study provide a new reference for improving the fruit color of Cerasus humilis in the future.
Differential Regulation of Anthocyanins in Cerasus humilis Fruit Color Revealed by Combined Transcriptome and Metabolome Analysis
Coloring is an important appearance quality of fruit. In order to evaluate the relationship between metabolites and fruit color, we analyzed the metabolites and transcriptional profiles of two different Cerasus humilis cultivars: “RF” (cv. Zhangwu, red fruit) and “YF” (cv. Nongda No.5, yellow fruit). The results of identification and quantification of metabolites showed that there were significant differences in the contents of 11 metabolites between RF and YF. Transcriptomics was used to analyze the expression patterns of genes related to the anthocyanin biosynthesis pathway, and subsequently, the regulation network of anthocyanin biosynthesis was established to explore their relationship with color formation. QRT-PCR, performed for 12 key genes, showed that the expression profiles of the differentially expressed genes were consistent with the results of the transcriptome data. A co-expression analysis revealed that the late genes were significantly positively correlated with most of the different metabolites. The results of the study provide a new reference for improving the fruit color of Cerasus humilis in the future.
Differential Regulation of Anthocyanins in Cerasus humilis Fruit Color Revealed by Combined Transcriptome and Metabolome Analysis
Xiaolong Ji (Autor:in) / Jing Ren (Autor:in) / Shaoyu Lang (Autor:in) / Di Wang (Autor:in) / Liwei Zhu (Autor:in) / Xingshun Song (Autor:in)
2020
Aufsatz (Zeitschrift)
Elektronische Ressource
Unbekannt
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