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EIF4A3 modulated circ_000999 promotes epithelial-mesenchymal transition in cadmium-induced malignant transformation through the miR-205-5p/ZEB1 axis
Graphical abstract Display Omitted
Highlights The high expression of circ_000999 facilitated the occurrence of EMT in cadmium-induced malignant transformation. Circ_000999 promoted EMT in Cd-induced malignant transformation of 16HBE cells through miR-205-5p/ZEB1 axis. EIF4A3 interacted with the parental gene AGTPBP1 to regulate high expression of circ_000999 in the process ofCd-induced EMT.
Abstract Cadmium (Cd) is an accumulative toxic metal which poses a serious threat to human health, even in trace amounts. One of the most important steps in the pathophysiology of lung cancer (LC) is the epithelial-mesenchymal transition (EMT). In this investigation, a cell malignant transformation model was established by exposing human bronchial epithelial cells (16HBE) to a low dose of Cd for 30 weeks, after which a highly expressed circular RNA (circ_000999) was identified. Cd-induced EMT was clearly observed in rat lungs and 16HBE cells, which was further enhanced following circ_000999-overexpression. Furthermore, upregulated EIF4A3 interacted with the parental gene AGTPBP1 to promote high expression of circ_000999. Subsequent experiments confirmed that circ_000999 could regulate the EMT process by competitively binding miR-205-5p and inhibiting its activity, consequently upregulating expression of zinc finger E-box binding protein 1 (ZEB1). Importantly, the circ_000999 expression level in LC tissues was significantly increased, exhibiting a strong correlation with EMT indicators. Overall, these findings provide a new objective and research direction for reversing lung EMT and subsequent treatment and prevention of LC.
EIF4A3 modulated circ_000999 promotes epithelial-mesenchymal transition in cadmium-induced malignant transformation through the miR-205-5p/ZEB1 axis
Graphical abstract Display Omitted
Highlights The high expression of circ_000999 facilitated the occurrence of EMT in cadmium-induced malignant transformation. Circ_000999 promoted EMT in Cd-induced malignant transformation of 16HBE cells through miR-205-5p/ZEB1 axis. EIF4A3 interacted with the parental gene AGTPBP1 to regulate high expression of circ_000999 in the process ofCd-induced EMT.
Abstract Cadmium (Cd) is an accumulative toxic metal which poses a serious threat to human health, even in trace amounts. One of the most important steps in the pathophysiology of lung cancer (LC) is the epithelial-mesenchymal transition (EMT). In this investigation, a cell malignant transformation model was established by exposing human bronchial epithelial cells (16HBE) to a low dose of Cd for 30 weeks, after which a highly expressed circular RNA (circ_000999) was identified. Cd-induced EMT was clearly observed in rat lungs and 16HBE cells, which was further enhanced following circ_000999-overexpression. Furthermore, upregulated EIF4A3 interacted with the parental gene AGTPBP1 to promote high expression of circ_000999. Subsequent experiments confirmed that circ_000999 could regulate the EMT process by competitively binding miR-205-5p and inhibiting its activity, consequently upregulating expression of zinc finger E-box binding protein 1 (ZEB1). Importantly, the circ_000999 expression level in LC tissues was significantly increased, exhibiting a strong correlation with EMT indicators. Overall, these findings provide a new objective and research direction for reversing lung EMT and subsequent treatment and prevention of LC.
EIF4A3 modulated circ_000999 promotes epithelial-mesenchymal transition in cadmium-induced malignant transformation through the miR-205-5p/ZEB1 axis
Wang, Donglei (Autor:in) / Chen, Shijie (Autor:in) / Shao, Yueqing (Autor:in) / Deng, Yang (Autor:in) / Huang, Lihua (Autor:in)
10.04.2024
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
| Wiley | 2024
| Wiley | 2024