Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Copper nanoparticles and copper ions promote horizontal transfer of plasmid-mediated multi-antibiotic resistance genes across bacterial genera
Abstract The spread of antibiotic resistance has become a major concern for public health. As emerging contaminants, various metallic nanoparticles (NPs) and ionic heavy metals have been ubiquitously detected in various environments. Although previous studies have indicated NPs and ionic heavy metals could exhibit co-selection effects for antibiotic resistance, little is known about whether and how they could promote antibiotic resistance spread via horizontal gene transfer across bacterial genera. This study, we report both CuO NPs and copper ions (Cu2+) could stimulate the conjugative transfer of multiple-drug resistance genes. When exposing bacteria to CuO NPs or Cu2+ at environmental-relevant and sub-inhibitory concentrations (e.g., 1–100 μmol/L), conjugation frequencies of plasmid-encoded antibiotic resistance genes across genera (i.e., from Escherichia coli to Pseudomonas putida) were significantly enhanced (p < 0.05). The over-production of reactive oxygen species played a crucial role in promoting conjugative transfer. Genome-wide RNA and protein sequencing suggested expressional levels of genes and proteins related to oxidative stress, cell membrane permeability, and pilus generation were significantly up-regulated under CuO NPs and Cu2+ exposure (p < 0.05). This study provides insights in the contributions of NPs and heavy metals on the spread of antibiotic resistance.
Graphical abstract Display Omitted
Highlights Both CuO NPs and Cu2+ facilitated conjugative transfer of antibiotic resistance. Exposure of CuO NPs or Cu2+ increased reactive oxygen species (ROS) production. The over-production of ROS played a crucial role in promoting conjugative transfer. Genes and proteins of oxidative stress were up-regulated under exposure of CuO NPs or Cu2+.
Copper nanoparticles and copper ions promote horizontal transfer of plasmid-mediated multi-antibiotic resistance genes across bacterial genera
Abstract The spread of antibiotic resistance has become a major concern for public health. As emerging contaminants, various metallic nanoparticles (NPs) and ionic heavy metals have been ubiquitously detected in various environments. Although previous studies have indicated NPs and ionic heavy metals could exhibit co-selection effects for antibiotic resistance, little is known about whether and how they could promote antibiotic resistance spread via horizontal gene transfer across bacterial genera. This study, we report both CuO NPs and copper ions (Cu2+) could stimulate the conjugative transfer of multiple-drug resistance genes. When exposing bacteria to CuO NPs or Cu2+ at environmental-relevant and sub-inhibitory concentrations (e.g., 1–100 μmol/L), conjugation frequencies of plasmid-encoded antibiotic resistance genes across genera (i.e., from Escherichia coli to Pseudomonas putida) were significantly enhanced (p < 0.05). The over-production of reactive oxygen species played a crucial role in promoting conjugative transfer. Genome-wide RNA and protein sequencing suggested expressional levels of genes and proteins related to oxidative stress, cell membrane permeability, and pilus generation were significantly up-regulated under CuO NPs and Cu2+ exposure (p < 0.05). This study provides insights in the contributions of NPs and heavy metals on the spread of antibiotic resistance.
Graphical abstract Display Omitted
Highlights Both CuO NPs and Cu2+ facilitated conjugative transfer of antibiotic resistance. Exposure of CuO NPs or Cu2+ increased reactive oxygen species (ROS) production. The over-production of ROS played a crucial role in promoting conjugative transfer. Genes and proteins of oxidative stress were up-regulated under exposure of CuO NPs or Cu2+.
Copper nanoparticles and copper ions promote horizontal transfer of plasmid-mediated multi-antibiotic resistance genes across bacterial genera
Zhang, Shuai (Autor:in) / Wang, Yue (Autor:in) / Song, Hailiang (Autor:in) / Lu, Ji (Autor:in) / Yuan, Zhiguo (Autor:in) / Guo, Jianhua (Autor:in)
Environmental International ; 129 ; 478-487
21.05.2019
10 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
| DOAJ | 2020