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Method for solidifying sand by utilizing halotolerant bacteria
The invention relates to a method for solidifying sand by utilizing halotolerant bacteria. The method selects Sporosarcina pasteurii as bacteria, the strain comes from American Type Culture Collection (ATCC), and the collection number is ATCC 11859. The method comprises the following steps: putting the strain in a nutrient broth medium to obtain a bacterial solution after domestication and enrichment; putting the bacterial solution in desert underground brine containing urea, calcium ions and salinity, and culturing to obtain a bacterial solution with an induced solidification effect; spraying the bacterial solution on the surface of sand soil; putting the sand soil of which the surface is sprayed with the bacterial solution in the natural drying environment for culturing; and naturally air-drying, so that the bacteria can utilize the desert underground brine to cement and solidify sand grains on the surface of the sand soil through the solidification effect so as to prevent the sand soil on the surface layer from migrating under the wind effect. The method is environment-friendly, is low in cost, and can be widely used for solidifying mobile and semi-mobile dunes.
Method for solidifying sand by utilizing halotolerant bacteria
The invention relates to a method for solidifying sand by utilizing halotolerant bacteria. The method selects Sporosarcina pasteurii as bacteria, the strain comes from American Type Culture Collection (ATCC), and the collection number is ATCC 11859. The method comprises the following steps: putting the strain in a nutrient broth medium to obtain a bacterial solution after domestication and enrichment; putting the bacterial solution in desert underground brine containing urea, calcium ions and salinity, and culturing to obtain a bacterial solution with an induced solidification effect; spraying the bacterial solution on the surface of sand soil; putting the sand soil of which the surface is sprayed with the bacterial solution in the natural drying environment for culturing; and naturally air-drying, so that the bacteria can utilize the desert underground brine to cement and solidify sand grains on the surface of the sand soil through the solidification effect so as to prevent the sand soil on the surface layer from migrating under the wind effect. The method is environment-friendly, is low in cost, and can be widely used for solidifying mobile and semi-mobile dunes.
Method for solidifying sand by utilizing halotolerant bacteria
PAN XIANGLIANG (Autor:in) / CHEN FEI (Autor:in) / SONG WENJUAN (Autor:in)
30.12.2015
Patent
Elektronische Ressource
Englisch
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