Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Method for inducing calcium carbonate precipitation to uniformly cover recycled coarse aggregate by bacillus pasteurii DSM33
The invention provides a method for inducing calcium carbonate precipitation to uniformly cover recycled coarse aggregate by bacillus pasteurii DSM33. The method comprises the steps of firstly, preparing a microorganism culture medium and a calcium source solution; secondly, drying the recycled coarse aggregate at low temperature, and soaking the recycled coarse aggregate in the microorganism culture medium to enable the recycled coarse aggregate to absorb enough microorganisms; thirdly, taking out the recycled coarse aggregate from the microorganism culture medium, and putting the recycled coarse aggregate into a sterile precipitation culture medium; then, equally dividing the solution containing the calcium source into a plurality of parts, and gradually adding the equally divided calcium source solution into the precipitation culture medium day by day; and finally, taking out the recycled coarse aggregate, and placing the recycled coarse aggregate in the atmosphere for air drying treatment. The method is clear and definite in operation steps, accords with engineering practice, efficiently utilizes calcium carbonate precipitates generated by microorganisms, enables the calcium carbonate precipitates to be uniformly attached to the surface of the recycled coarse aggregate, fills and covers pores and old mortar on the surface of the recycled coarse aggregate, and finally remarkably reduces the water absorption rate of the recycled coarse aggregate. The compressive strength of the recycled aggregate concrete is improved.
本发明提供了一种巴氏芽孢杆菌DSM33诱导碳酸钙沉淀均匀覆盖再生粗骨料的方法。首先，配置微生物培养基和钙源溶液；其次，低温烘干再生粗骨料，并将其浸泡于微生物培养基中，使其吸收足够多的微生物；再次，将再生粗骨料从微生物培养基中取出，放置于一个无菌的沉淀培养基中；接着，将含有钙源的溶液均分为若干份，将均分后的钙源溶液逐次逐天加入沉淀培养基中；最后，将再生粗骨料取出，并放置于大气中风干处理。本发明操作步骤清晰明确，符合工程实际，高效地利用了微生物所产生的碳酸钙沉淀，使其均匀地附着在再生粗骨料表面，填补和覆盖了再生粗骨料表面的孔隙和老砂浆，最终显著地降低了再生粗骨料的吸水率，提升了再生骨料混凝土的抗压强度。
Method for inducing calcium carbonate precipitation to uniformly cover recycled coarse aggregate by bacillus pasteurii DSM33
The invention provides a method for inducing calcium carbonate precipitation to uniformly cover recycled coarse aggregate by bacillus pasteurii DSM33. The method comprises the steps of firstly, preparing a microorganism culture medium and a calcium source solution; secondly, drying the recycled coarse aggregate at low temperature, and soaking the recycled coarse aggregate in the microorganism culture medium to enable the recycled coarse aggregate to absorb enough microorganisms; thirdly, taking out the recycled coarse aggregate from the microorganism culture medium, and putting the recycled coarse aggregate into a sterile precipitation culture medium; then, equally dividing the solution containing the calcium source into a plurality of parts, and gradually adding the equally divided calcium source solution into the precipitation culture medium day by day; and finally, taking out the recycled coarse aggregate, and placing the recycled coarse aggregate in the atmosphere for air drying treatment. The method is clear and definite in operation steps, accords with engineering practice, efficiently utilizes calcium carbonate precipitates generated by microorganisms, enables the calcium carbonate precipitates to be uniformly attached to the surface of the recycled coarse aggregate, fills and covers pores and old mortar on the surface of the recycled coarse aggregate, and finally remarkably reduces the water absorption rate of the recycled coarse aggregate. The compressive strength of the recycled aggregate concrete is improved.
本发明提供了一种巴氏芽孢杆菌DSM33诱导碳酸钙沉淀均匀覆盖再生粗骨料的方法。首先，配置微生物培养基和钙源溶液；其次，低温烘干再生粗骨料，并将其浸泡于微生物培养基中，使其吸收足够多的微生物；再次，将再生粗骨料从微生物培养基中取出，放置于一个无菌的沉淀培养基中；接着，将含有钙源的溶液均分为若干份，将均分后的钙源溶液逐次逐天加入沉淀培养基中；最后，将再生粗骨料取出，并放置于大气中风干处理。本发明操作步骤清晰明确，符合工程实际，高效地利用了微生物所产生的碳酸钙沉淀，使其均匀地附着在再生粗骨料表面，填补和覆盖了再生粗骨料表面的孔隙和老砂浆，最终显著地降低了再生粗骨料的吸水率，提升了再生骨料混凝土的抗压强度。
Method for inducing calcium carbonate precipitation to uniformly cover recycled coarse aggregate by bacillus pasteurii DSM33
一种巴氏芽孢杆菌DSM33诱导碳酸钙沉淀均匀覆盖再生粗骨料的方法
ZHAO YUXI (Autor:in) / ZENG WEILAI (Autor:in) / FENG ZHANGYAO (Autor:in)
04.12.2020
Patent
Elektronische Ressource
Chinesisch
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