Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Flexible and high throughput sequencing of target genomic regions
The present disclosure relates to materials and methods for capturing a target genomic region, comprising hybridizing an extension probe and a ligation probe to a target sequence on either side of the target genomic region; extending the 3'end of the extension probe until the 3 'end of the extended extension probe is adjacent to the 5' end of the connection probe; the 3'end of the elongated extension probe is connected to the 5 'end of the connection probe to create a knotted probe. The binding probe may be PCR amplified to produce copies of a target genomic region that may be detected or sequenced. Certain embodiments of the invention also provide methods of producing a double-stranded probe suitable for capturing and analyzing two strands of a target genomic region in double-stranded genomic DNA. The invention also provides kits for performing the methods disclosed herein.
本公开涉及用于捕获靶基因组区域的材料和方法，包括将延伸探针和连接探针与靶基因组区域两侧的靶序列杂交；将延伸探针的3'端延长，直到延长的延伸探针的3'端与连接探针的5'端相邻；将延长的延伸探针的3'端与连接探针的5'端连接以产生扎结探针。可以对扎结探针进行PCR扩增，以产生可以检测或测序的靶基因组区域的拷贝。本发明的某些实施方式还提供了生产适用于捕获和分析双链基因组DNA中靶基因组区域的两条链的双链探针的方法。本发明还提供用于执行本文公开的方法的试剂盒。
Flexible and high throughput sequencing of target genomic regions
The present disclosure relates to materials and methods for capturing a target genomic region, comprising hybridizing an extension probe and a ligation probe to a target sequence on either side of the target genomic region; extending the 3'end of the extension probe until the 3 'end of the extended extension probe is adjacent to the 5' end of the connection probe; the 3'end of the elongated extension probe is connected to the 5 'end of the connection probe to create a knotted probe. The binding probe may be PCR amplified to produce copies of a target genomic region that may be detected or sequenced. Certain embodiments of the invention also provide methods of producing a double-stranded probe suitable for capturing and analyzing two strands of a target genomic region in double-stranded genomic DNA. The invention also provides kits for performing the methods disclosed herein.
本公开涉及用于捕获靶基因组区域的材料和方法，包括将延伸探针和连接探针与靶基因组区域两侧的靶序列杂交；将延伸探针的3'端延长，直到延长的延伸探针的3'端与连接探针的5'端相邻；将延长的延伸探针的3'端与连接探针的5'端连接以产生扎结探针。可以对扎结探针进行PCR扩增，以产生可以检测或测序的靶基因组区域的拷贝。本发明的某些实施方式还提供了生产适用于捕获和分析双链基因组DNA中靶基因组区域的两条链的双链探针的方法。本发明还提供用于执行本文公开的方法的试剂盒。
Flexible and high throughput sequencing of target genomic regions
靶基因组区域的灵活且高通量的测序
GOMIDE NEVES LEANDRO (Autor:in) / PAYTON ADAM (Autor:in)
18.03.2022
Patent
Elektronische Ressource
Chinesisch
IPC:
E04C
STRUCTURAL ELEMENTS
,
Bauelemente
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