Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Application method of nanoparticle-coated microorganisms in concrete crack self-repairing
The invention provides an application method of nanoparticles-coated microorganisms in concrete crack self-repairing, which comprises the following steps: step 1, preparing nutritional cells, and preparing a solid culture medium; step 2, preparing spores; the method comprises the following steps: inoculating Bacillus sp.B6 into a culture solution of an LB culture medium by using a culture solution culture method, and then transferring the Bacillus sp.B6 into a culture solution for promoting spores to form an MSP culture medium; 3, spores are diluted, and qualified bacteria concentration is selected; step 4, preparing nano particles, and selecting nano tin dioxide particles; step 5, combining the spores with the nano material; forming a mixed suspension; step 6, preparing self-repairing small balls; the prepared concrete self-repairing agent is small in microsphere size, large in specific surface area and higher in interaction capacity with microorganisms, the novel nanometer material has high catalytic activity, adsorption and catalytic reaction are prone to occurring on the surface of the novel nanometer material, and the survival rate of the microorganisms in concrete can be effectively increased when the novel nanometer material is attached to the surfaces of the microorganisms.
本发明提供了一种纳米粒子包覆的微生物在混凝土裂缝自修复中的应用方法，步骤1，制备营养细胞，配置固体培养基；步骤2，制备芽孢；使用培养液培养法，将Bacillus sp.B6芽孢杆菌接种于LB培养基的培养液中，再转接于促芽孢形成MSP培养基的培养液中；步骤3，稀释芽孢，选取合格细菌浓度；步骤4，准备纳米颗粒，选取纳米二氧化锡颗粒；步骤5，芽孢和纳米材料的结合；形成混合悬浮液；步骤6，制备自修复小球；本发明制备的混凝土自修复剂微球尺寸小、比表面积大、与微生物互作能力更强，而且新型纳米材料具有高催化活性，其表面容易发生吸附和催化反应，附着在微生物表面能有效提高微生物在混凝土中的存活率。
Application method of nanoparticle-coated microorganisms in concrete crack self-repairing
The invention provides an application method of nanoparticles-coated microorganisms in concrete crack self-repairing, which comprises the following steps: step 1, preparing nutritional cells, and preparing a solid culture medium; step 2, preparing spores; the method comprises the following steps: inoculating Bacillus sp.B6 into a culture solution of an LB culture medium by using a culture solution culture method, and then transferring the Bacillus sp.B6 into a culture solution for promoting spores to form an MSP culture medium; 3, spores are diluted, and qualified bacteria concentration is selected; step 4, preparing nano particles, and selecting nano tin dioxide particles; step 5, combining the spores with the nano material; forming a mixed suspension; step 6, preparing self-repairing small balls; the prepared concrete self-repairing agent is small in microsphere size, large in specific surface area and higher in interaction capacity with microorganisms, the novel nanometer material has high catalytic activity, adsorption and catalytic reaction are prone to occurring on the surface of the novel nanometer material, and the survival rate of the microorganisms in concrete can be effectively increased when the novel nanometer material is attached to the surfaces of the microorganisms.
本发明提供了一种纳米粒子包覆的微生物在混凝土裂缝自修复中的应用方法，步骤1，制备营养细胞，配置固体培养基；步骤2，制备芽孢；使用培养液培养法，将Bacillus sp.B6芽孢杆菌接种于LB培养基的培养液中，再转接于促芽孢形成MSP培养基的培养液中；步骤3，稀释芽孢，选取合格细菌浓度；步骤4，准备纳米颗粒，选取纳米二氧化锡颗粒；步骤5，芽孢和纳米材料的结合；形成混合悬浮液；步骤6，制备自修复小球；本发明制备的混凝土自修复剂微球尺寸小、比表面积大、与微生物互作能力更强，而且新型纳米材料具有高催化活性，其表面容易发生吸附和催化反应，附着在微生物表面能有效提高微生物在混凝土中的存活率。
Application method of nanoparticle-coated microorganisms in concrete crack self-repairing
纳米粒子包覆的微生物在混凝土裂缝自修复中的应用方法
TAN PEIWEN (Autor:in) / DENG XU (Autor:in) / AHMED MUHAMMAD ASLAN (Autor:in) / ZHANG JINLONG (Autor:in) / LIU BING (Autor:in) / FANG DOU (Autor:in)
19.11.2024
Patent
Elektronische Ressource
Chinesisch
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