Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Alpha-phellandrene-induced apoptosis in mice leukemia WEHI-3 cells in vitro
Although reports have shown that [alpha]-phellandrene ([alpha]-PA) is one of the monoterpenes and is often used in the food and perfume industry, our previous studies have indicated that [alpha]-PA promoted immune responses in normal mice in vivo. However, there is no available information to show that [alpha]-PA induced cell apoptosis in cancer cells, thus, we investigated the effects of [alpha]-PA on the cell morphology, viability, cell cycle distribution, and apoptosis in mice leukemia WEHI-3 cells in vitro. Results indicated that [alpha]-PA induced cell morphological changes and decreased viability, induced G0/G1 arrest and sub-G1 phase (apoptosis) in WEHI-3 cells. [alpha]-PA increased the productions of reactive oxygen species (ROS) and Ca2+ and decreased the levels of mitochondrial membrane potential ([Delta]Ψm) in dose- and time-dependent manners in WEHI-3 cells that were analyzed by flow cytometer. Results from confocal laser microscopic system examinations show that [alpha]-PA promoted the release of cytochrome c, AIF, and Endo G from mitochondria in WEHI-3 cells. These results are the first findings to provide new information for understanding the mechanisms by which [alpha]-PA induces cell cycle arrest and apoptosis in WEHI-3 cells in vitro. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1640-1651, 2016.
Alpha-phellandrene-induced apoptosis in mice leukemia WEHI-3 cells in vitro
Although reports have shown that [alpha]-phellandrene ([alpha]-PA) is one of the monoterpenes and is often used in the food and perfume industry, our previous studies have indicated that [alpha]-PA promoted immune responses in normal mice in vivo. However, there is no available information to show that [alpha]-PA induced cell apoptosis in cancer cells, thus, we investigated the effects of [alpha]-PA on the cell morphology, viability, cell cycle distribution, and apoptosis in mice leukemia WEHI-3 cells in vitro. Results indicated that [alpha]-PA induced cell morphological changes and decreased viability, induced G0/G1 arrest and sub-G1 phase (apoptosis) in WEHI-3 cells. [alpha]-PA increased the productions of reactive oxygen species (ROS) and Ca2+ and decreased the levels of mitochondrial membrane potential ([Delta]Ψm) in dose- and time-dependent manners in WEHI-3 cells that were analyzed by flow cytometer. Results from confocal laser microscopic system examinations show that [alpha]-PA promoted the release of cytochrome c, AIF, and Endo G from mitochondria in WEHI-3 cells. These results are the first findings to provide new information for understanding the mechanisms by which [alpha]-PA induces cell cycle arrest and apoptosis in WEHI-3 cells in vitro. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1640-1651, 2016.
Alpha-phellandrene-induced apoptosis in mice leukemia WEHI-3 cells in vitro
Jen-Jyh Lin (Autor:in) / Shu-Chun Hsu / Kung-Wen Lu / Yi-Shih Ma / Chih-Chung Wu / Hsu-Feng Lu / Jaw-Chyun Chen / Jaung-Geng Lin / Ping-Ping Wu / Jing-Gung Chung
2016
Aufsatz (Zeitschrift)
Englisch