Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Horseradish peroxidase-assisted approach to decolorize and detoxify dye pollutants in a packed bed bioreactor
In this study, horseradish peroxidase (HRP) was covalently immobilized on the calcium-alginate support using glutaraldehyde (GA) as a cross-linking reagent for detoxification and degradation of synthetic dyes. Immobilization procedure furnished significant immobilization efficiency (86.27 [+ or -] 3.43%) along with apparent and relative activity of 24.39 [+ or -] 1.03 U/g and 84.97 [+ or -] 3.54%, respectively, for immobilized-HRP. In comparison to free-state, immobilized-HRP catalyzed the substrate oxidation reaction in a slightly acidic and wider temperature range, with an optimum at 60 [degrees]C. After 10 and 60 min of incubation at 60 [degrees]C, the immobilized-HRP displayed 99.0% and 89.0% of residual activities, whereas the free counterpart retained only 34.0% and 18.0% of residual activities, respectively. Moreover, the immobilized-HRP showed potential efficiency for the decolorization of dyes in sequential dye-decolorizing batch reactions. Cytotoxicity analysis using a plant bioassay and acute test demonstrated that the Ca-alginate immobilized-HRP may effectively be used for detoxification of dyes and has a great potential for large-scale environmental remediation.
Horseradish peroxidase-assisted approach to decolorize and detoxify dye pollutants in a packed bed bioreactor
In this study, horseradish peroxidase (HRP) was covalently immobilized on the calcium-alginate support using glutaraldehyde (GA) as a cross-linking reagent for detoxification and degradation of synthetic dyes. Immobilization procedure furnished significant immobilization efficiency (86.27 [+ or -] 3.43%) along with apparent and relative activity of 24.39 [+ or -] 1.03 U/g and 84.97 [+ or -] 3.54%, respectively, for immobilized-HRP. In comparison to free-state, immobilized-HRP catalyzed the substrate oxidation reaction in a slightly acidic and wider temperature range, with an optimum at 60 [degrees]C. After 10 and 60 min of incubation at 60 [degrees]C, the immobilized-HRP displayed 99.0% and 89.0% of residual activities, whereas the free counterpart retained only 34.0% and 18.0% of residual activities, respectively. Moreover, the immobilized-HRP showed potential efficiency for the decolorization of dyes in sequential dye-decolorizing batch reactions. Cytotoxicity analysis using a plant bioassay and acute test demonstrated that the Ca-alginate immobilized-HRP may effectively be used for detoxification of dyes and has a great potential for large-scale environmental remediation.
Horseradish peroxidase-assisted approach to decolorize and detoxify dye pollutants in a packed bed bioreactor
Bilal, Muhammad (Autor:in) / Iqbal, Hafiz M.N / Hussain Shah, Syed Zakir / Hu, Hongbo / Wang, Wei / Zhang, Xuehong
2016
Aufsatz (Zeitschrift)
Englisch
BKL:
43.00
| DOAJ | 2024
Horseradish peroxidase-modified porous silicon for phenol monitoring
| British Library Online Contents | 2013
Techniques Galore: How to Select the Best One to Detoxify Contaminants?
| American Chemical Society | 2021
Enzymatic Removal of Phenolic Compound from Real Industrial Wastewaters Using Horseradish Peroxidase
| British Library Conference Proceedings | 1995