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The optimization of biohydrogen production by bacteria using residual glycerol from biodiesel synthesis
In this research the production of hydrogen by Klebsiella pneumoniae BLb01 using residual glycerol discharged from a biodiesel fuel production plant was investigated. Klebsiella pneumoniae BLb01 was isolated from a bacteria-rich sludge of an upflow anaerobic sludge blanket reactor (UASB) of a soybean processing plant. A Plackett–Burman design (P-B) and Response Surface Methodology (RSM) were employed to determine the optimal condition for enhanced hydrogen production. The maximal hydrogen production, which was 45.0 mol % and with 98% of glycerol degradation, was achieved with the optimized medium with the following composition: 30 g L−1 glycerol; 3 g L−1 yeast ex tract 3 g L−1 K2HPO4; 1 g L−1 KH2PO4; temperature 39°C and pH 9.0. These results show the ability of this new strain of effectively converting residual glycerol into value-added energy products.
The optimization of biohydrogen production by bacteria using residual glycerol from biodiesel synthesis
In this research the production of hydrogen by Klebsiella pneumoniae BLb01 using residual glycerol discharged from a biodiesel fuel production plant was investigated. Klebsiella pneumoniae BLb01 was isolated from a bacteria-rich sludge of an upflow anaerobic sludge blanket reactor (UASB) of a soybean processing plant. A Plackett–Burman design (P-B) and Response Surface Methodology (RSM) were employed to determine the optimal condition for enhanced hydrogen production. The maximal hydrogen production, which was 45.0 mol % and with 98% of glycerol degradation, was achieved with the optimized medium with the following composition: 30 g L−1 glycerol; 3 g L−1 yeast ex tract 3 g L−1 K2HPO4; 1 g L−1 KH2PO4; temperature 39°C and pH 9.0. These results show the ability of this new strain of effectively converting residual glycerol into value-added energy products.
The optimization of biohydrogen production by bacteria using residual glycerol from biodiesel synthesis
Costa, Janaina B. (Autor:in) / Rossi, Daniele M. (Autor:in) / De Souza, Elisangela A. (Autor:in) / Samios, Dimitrios (Autor:in) / Bregalda, Fernanda (Autor:in) / do Carmo Ruaro Peralba, Maria (Autor:in) / Flores, Simone H. (Autor:in) / Ayub, Marco Antônio Z. (Autor:in)
Journal of Environmental Science and Health, Part A ; 46 ; 1461-1468
01.11.2011
8 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Unbekannt
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