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Enzymatic-assisted polymerization of the lignin obtained from a macroalgae consortium, using an extracellular laccase-like enzyme (Tg-laccase) from Tetraselmis gracilis
In the past decade, Mexican coasts have received an enormous influx of macroalgae species, producing serious environmental and public health concerns. Here, we developed a green methodology to generate a new polymer from the lignin contained in the macroalgae. The methodology consists in lignin extraction-by-boiling and its subsequent polymerization with a laccase-like enzyme from the green algae Tetraselmis gracilis (Tg-laccase). Mass spectrometry revealed the presence of guaiacyl (G), p-hydroxyphenyl (H), and sinapyl alcohol as the main monolignols in the lignin from Sargassum sp. On the other hand, MALDI-TOF spectra shows an increase in the size of the lignin chain after enzymatic polymerization process with Tg-laccase. Besides, the characterization of the novel polymer -using 1H NMR, FTIR, SEC-FPLC, and UV/Vis- allowed establishing that during the polymerization process there is a decrease in the number of phenolic groups as well as loss of aromatic protons, which allowed proposing a polimerizacion mechanism. This methodology could be promising in the development of a new lignin-based polymer and would open a new direction for the environmental management of the macroalgae on the Mexican beaches.
Enzymatic-assisted polymerization of the lignin obtained from a macroalgae consortium, using an extracellular laccase-like enzyme (Tg-laccase) from Tetraselmis gracilis
In the past decade, Mexican coasts have received an enormous influx of macroalgae species, producing serious environmental and public health concerns. Here, we developed a green methodology to generate a new polymer from the lignin contained in the macroalgae. The methodology consists in lignin extraction-by-boiling and its subsequent polymerization with a laccase-like enzyme from the green algae Tetraselmis gracilis (Tg-laccase). Mass spectrometry revealed the presence of guaiacyl (G), p-hydroxyphenyl (H), and sinapyl alcohol as the main monolignols in the lignin from Sargassum sp. On the other hand, MALDI-TOF spectra shows an increase in the size of the lignin chain after enzymatic polymerization process with Tg-laccase. Besides, the characterization of the novel polymer -using 1H NMR, FTIR, SEC-FPLC, and UV/Vis- allowed establishing that during the polymerization process there is a decrease in the number of phenolic groups as well as loss of aromatic protons, which allowed proposing a polimerizacion mechanism. This methodology could be promising in the development of a new lignin-based polymer and would open a new direction for the environmental management of the macroalgae on the Mexican beaches.
Enzymatic-assisted polymerization of the lignin obtained from a macroalgae consortium, using an extracellular laccase-like enzyme (Tg-laccase) from Tetraselmis gracilis
Antúnez-Argüelles, Erika (Autor:in) / Herrera-Bulnes, Marlo (Autor:in) / Torres-Ariño, Alejandra (Autor:in) / Mirón-Enríquez, Coral (Autor:in) / Soriano-García, Manuel (Autor:in) / Robles-Gómez, Edson (Autor:in)
Journal of Environmental Science and Health, Part A ; 55 ; 739-747
11.05.2020
9 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
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