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Genome Editing VEGFA Prevents Corneal Neovascularization In Vivo
AbstractCorneal neovascularization (CNV) is a common clinical finding seen in a range of eye diseases. Current therapeutic approaches to treat corneal angiogenesis, in which vascular endothelial growth factor (VEGF) A plays a central role, can cause a variety of adverse side effects. The technology of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 can edit VEGFA gene to suppress its expression. CRISPR offers a novel opportunity to treat CNV. This study shows that depletion of VEGFA with a novel CRISPR/Cas9 system inhibits proliferation, migration, and tube formation of human umbilical vein endothelial cells (HUVECs) in vitro. Importantly, subconjunctival injection of this dual AAV‐SpCas9/sgRNA‐VEGFA system is demonstrated which blocks suture‐induced expression of VEGFA, CD31, and α‐smooth muscle actin as well as corneal neovascularization in mice. This study has established a strong foundation for the treatment of corneal neovascularization via a gene editing approach for the first time.
Genome Editing VEGFA Prevents Corneal Neovascularization In Vivo
AbstractCorneal neovascularization (CNV) is a common clinical finding seen in a range of eye diseases. Current therapeutic approaches to treat corneal angiogenesis, in which vascular endothelial growth factor (VEGF) A plays a central role, can cause a variety of adverse side effects. The technology of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 can edit VEGFA gene to suppress its expression. CRISPR offers a novel opportunity to treat CNV. This study shows that depletion of VEGFA with a novel CRISPR/Cas9 system inhibits proliferation, migration, and tube formation of human umbilical vein endothelial cells (HUVECs) in vitro. Importantly, subconjunctival injection of this dual AAV‐SpCas9/sgRNA‐VEGFA system is demonstrated which blocks suture‐induced expression of VEGFA, CD31, and α‐smooth muscle actin as well as corneal neovascularization in mice. This study has established a strong foundation for the treatment of corneal neovascularization via a gene editing approach for the first time.
Genome Editing VEGFA Prevents Corneal Neovascularization In Vivo
Advanced Science
Zeng, Zhenhai (Autor:in) / Li, Siheng (Autor:in) / Ye, Xiuhong (Autor:in) / Wang, Yiran (Autor:in) / Wang, Qinmei (Autor:in) / Chen, Zhongxing (Autor:in) / Wang, Ziqian (Autor:in) / Zhang, Jun (Autor:in) / Wang, Qing (Autor:in) / Chen, Lu (Autor:in)
Advanced Science ; 11
01.07.2024
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
Genome Editing VEGFA Prevents Corneal Neovascularization In Vivo (Adv. Sci. 25/2024)
Wiley | 2024
|Genome Editing VEGFA Prevents Corneal Neovascularization In Vivo (Adv. Sci. 25/2024)
Wiley | 2024
|