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Phage‐Inducible Chromosomal Islands as a Diagnostic Platform to Capture and Detect Bacterial Pathogens
https://orcid.org/0000-0002-9056-3055
https://orcid.org/0000-0002-6879-8405
https://orcid.org/0000-0002-6439-5262
https://orcid.org/0000-0002-2358-1050
AbstractPhage‐inducible chromosomal islands (PICIs) are a family of phage satellites that hijack phage components to facilitate their mobility and spread. Recently, these genetic constructs are repurposed as antibacterial drones, enabling a new toolbox for unorthodox applications in biotechnology. To illustrate a new suite of functions, the authors have developed a user‐friendly diagnostic system, based upon PICI transduction to selectively enrich bacteria, allowing the detection and sequential recovery of Escherichia coli and Staphylococcus aureus. The system enables high transfer rates and sensitivities in comparison with phages, with detection down to ≈50 CFU mL−1. In contrast to conventional detection strategies, which often rely on nucleic acid molecular assays, and cannot differentiate between dead and live organisms, this approach enables visual sensing of viable pathogens only, through the expression of a reporter gene encoded in the PICI. The approach extends diagnostic sensing mechanisms beyond cell‐free synthetic biology strategies, enabling new synthetic biology/biosensing toolkits.
Phage‐Inducible Chromosomal Islands as a Diagnostic Platform to Capture and Detect Bacterial Pathogens
https://orcid.org/0000-0002-9056-3055
https://orcid.org/0000-0002-6879-8405
https://orcid.org/0000-0002-6439-5262
https://orcid.org/0000-0002-2358-1050
AbstractPhage‐inducible chromosomal islands (PICIs) are a family of phage satellites that hijack phage components to facilitate their mobility and spread. Recently, these genetic constructs are repurposed as antibacterial drones, enabling a new toolbox for unorthodox applications in biotechnology. To illustrate a new suite of functions, the authors have developed a user‐friendly diagnostic system, based upon PICI transduction to selectively enrich bacteria, allowing the detection and sequential recovery of Escherichia coli and Staphylococcus aureus. The system enables high transfer rates and sensitivities in comparison with phages, with detection down to ≈50 CFU mL−1. In contrast to conventional detection strategies, which often rely on nucleic acid molecular assays, and cannot differentiate between dead and live organisms, this approach enables visual sensing of viable pathogens only, through the expression of a reporter gene encoded in the PICI. The approach extends diagnostic sensing mechanisms beyond cell‐free synthetic biology strategies, enabling new synthetic biology/biosensing toolkits.
Phage‐Inducible Chromosomal Islands as a Diagnostic Platform to Capture and Detect Bacterial Pathogens
https://orcid.org/0000-0002-9056-3055
https://orcid.org/0000-0002-6879-8405
https://orcid.org/0000-0002-6439-5262
https://orcid.org/0000-0002-2358-1050
AbstractPhage‐inducible chromosomal islands (PICIs) are a family of phage satellites that hijack phage components to facilitate their mobility and spread. Recently, these genetic constructs are repurposed as antibacterial drones, enabling a new toolbox for unorthodox applications in biotechnology. To illustrate a new suite of functions, the authors have developed a user‐friendly diagnostic system, based upon PICI transduction to selectively enrich bacteria, allowing the detection and sequential recovery of Escherichia coli and Staphylococcus aureus. The system enables high transfer rates and sensitivities in comparison with phages, with detection down to ≈50 CFU mL−1. In contrast to conventional detection strategies, which often rely on nucleic acid molecular assays, and cannot differentiate between dead and live organisms, this approach enables visual sensing of viable pathogens only, through the expression of a reporter gene encoded in the PICI. The approach extends diagnostic sensing mechanisms beyond cell‐free synthetic biology strategies, enabling new synthetic biology/biosensing toolkits.
Phage‐Inducible Chromosomal Islands as a Diagnostic Platform to Capture and Detect Bacterial Pathogens
Advanced Science
Ibarra‐Chávez, Rodrigo (Autor:in) / Reboud, Julien (Autor:in) / Penadés, José R. (Autor:in) / Cooper, Jonathan M. (Autor:in)
Advanced Science ; 10
01.08.2023
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
| Wiley | 2023
Pathogenicity islands and phage conversion: evolutionary aspects of bacterial pathogenesis
| British Library Online Contents | 2000
| British Library Online Contents | 2014
| British Library Online Contents | 2014