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Susceptibility of five strains of Cryptosporidium parvum oocysts to UV light
Previous evaluations of the effect of ultraviolet (UV) light on Cryptosporidium parvum oocysts have been limited to a single strain—the Iowa strain. This study investigated the response of five strains of C. parvum to UV. A collimated beam apparatus was used to apply controlled doses of monochromatic (254 nm) UV to oocysts of the Iowa, Moredun, Texas A&M, Maine, and Glasgow strains. Irradiation was measured using a calibrated radiometer and sensor. Inactivation was quantified through animal infectivity by inoculation of cohorts of CD‐1 neonatal mice with UV‐treated and untreated control oocysts of each strain followed by examination of intestinal sections for infection using hemotoxylin and eosin staining. A UV light dose of 10 mJ/cm2 achieved at least 4‐log10 inactivation of all strains evaluated. All five strains of C. parvum were shown to be highly susceptible to low levels of UV light.
Susceptibility of five strains of Cryptosporidium parvum oocysts to UV light
Previous evaluations of the effect of ultraviolet (UV) light on Cryptosporidium parvum oocysts have been limited to a single strain—the Iowa strain. This study investigated the response of five strains of C. parvum to UV. A collimated beam apparatus was used to apply controlled doses of monochromatic (254 nm) UV to oocysts of the Iowa, Moredun, Texas A&M, Maine, and Glasgow strains. Irradiation was measured using a calibrated radiometer and sensor. Inactivation was quantified through animal infectivity by inoculation of cohorts of CD‐1 neonatal mice with UV‐treated and untreated control oocysts of each strain followed by examination of intestinal sections for infection using hemotoxylin and eosin staining. A UV light dose of 10 mJ/cm2 achieved at least 4‐log10 inactivation of all strains evaluated. All five strains of C. parvum were shown to be highly susceptible to low levels of UV light.
Susceptibility of five strains of Cryptosporidium parvum oocysts to UV light
Clancy, Jennifer L. (Autor:in) / Marshall, Marilyn M. (Autor:in) / Hargy, Thomas M. (Autor:in) / Korich, Dick G. (Autor:in)
Journal ‐ American Water Works Association ; 96 ; 84-93
01.03.2004
10 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
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