Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Optimizing the Recovery of Surrogates for Bacterial Bioterrorism Agents from Ventilation Filters
10.1002/clen.200700182.abs
Heating, ventilating, and air‐conditioning (HVAC) filters have the potential to serve as long‐term integrated samplers for biological particles that may be released intentionally or unintentionally into a public building. Methods for eluting bacteria that are surrogates for potential bioterrorism agents from HVAC filters were tested for their ability to recover as high a percentage of culturable bacteria as possible. A solution of 0.02% Tween‐80 in water was an effective eluent forBacillus globigii (BG) spores whereas phosphate‐buffered saline was the most effective eluent for vegetative bacteria. Hand shaking was a more effective agitation method for high recovery rates than vortexing or mechanical shaking. The results indicate that BG spores can be eluted successfully while maintaining essentially 100% culturability. The recovery of the vegetative bacteriaMannheimia haemolytica andYersinia ruckeri was 80% or higher under optimal conditions. The culturability of the vegetative bacteria eluted from HVAC filters declined substantially within as little as 2 h after the bacteria were loaded onto the filter. The culturability of the BG spores eluted from filters exhibited an exponential decay subsequent to loading with a half‐life of 8–9 days.
Optimizing the Recovery of Surrogates for Bacterial Bioterrorism Agents from Ventilation Filters
10.1002/clen.200700182.abs
Heating, ventilating, and air‐conditioning (HVAC) filters have the potential to serve as long‐term integrated samplers for biological particles that may be released intentionally or unintentionally into a public building. Methods for eluting bacteria that are surrogates for potential bioterrorism agents from HVAC filters were tested for their ability to recover as high a percentage of culturable bacteria as possible. A solution of 0.02% Tween‐80 in water was an effective eluent forBacillus globigii (BG) spores whereas phosphate‐buffered saline was the most effective eluent for vegetative bacteria. Hand shaking was a more effective agitation method for high recovery rates than vortexing or mechanical shaking. The results indicate that BG spores can be eluted successfully while maintaining essentially 100% culturability. The recovery of the vegetative bacteriaMannheimia haemolytica andYersinia ruckeri was 80% or higher under optimal conditions. The culturability of the vegetative bacteria eluted from HVAC filters declined substantially within as little as 2 h after the bacteria were loaded onto the filter. The culturability of the BG spores eluted from filters exhibited an exponential decay subsequent to loading with a half‐life of 8–9 days.
Optimizing the Recovery of Surrogates for Bacterial Bioterrorism Agents from Ventilation Filters
Kim, Seung Won (Autor:in) / Raynor, Peter C. (Autor:in) / Kuehn, Thomas H. (Autor:in) / Goyal, Sagar M. (Autor:in) / Ramakrishnan, M. A. (Autor:in) / Anantharaman, Senthilvelan (Autor:in) / Farnsworth, James E. (Autor:in)
CLEAN – Soil, Air, Water ; 36 ; 601-608
01.07.2008
8 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
HVAC , Bioaerosol , Bioterrorism , Bacteria , Filters
Optimizing the Recovery of Surrogates for Bacterial Bioterrorism Agents from Ventilation Filters
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