Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Optimization of Polyhydroxybutyrate Production by Bacillus safensis EBT1
The present study deals with the enhanced production of polyhydroxybutyrate (PHB) using a novel potent strain. On the basis of morphological, biochemical, and 16S rRNA analysis, the strain was identified as Bacillus safensis EBT1. The presence of PHB in the cells was identified and affirmed using fluorescent microscopy and Fourier transform infrared spectroscopy. The molecular weight of PHB was ascertained by gel permeation chromatography. Under optimized conditions, B. safensis EBT1 showed maximum PHB production and growth with glucose as the carbon source, yeast extract as the nitrogen source, and sugarcane bagasse as the substrate. The process parameters were optimized using central composite design: pH 7, 45 °C and 48 h incubation time. At these conditions, highest biomass concentration of 7.54 g/L and PHB production of 6.41 g/L were observed.
Optimization of Polyhydroxybutyrate Production by Bacillus safensis EBT1
The present study deals with the enhanced production of polyhydroxybutyrate (PHB) using a novel potent strain. On the basis of morphological, biochemical, and 16S rRNA analysis, the strain was identified as Bacillus safensis EBT1. The presence of PHB in the cells was identified and affirmed using fluorescent microscopy and Fourier transform infrared spectroscopy. The molecular weight of PHB was ascertained by gel permeation chromatography. Under optimized conditions, B. safensis EBT1 showed maximum PHB production and growth with glucose as the carbon source, yeast extract as the nitrogen source, and sugarcane bagasse as the substrate. The process parameters were optimized using central composite design: pH 7, 45 °C and 48 h incubation time. At these conditions, highest biomass concentration of 7.54 g/L and PHB production of 6.41 g/L were observed.
Optimization of Polyhydroxybutyrate Production by Bacillus safensis EBT1
Madhumathi, Ramasamy (Autor:in) / Muthukumar, Karuppan (Autor:in) / Velan, Manickam (Autor:in)
CLEAN – Soil, Air, Water ; 44 ; 1066-1074
01.08.2016
10 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
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| British Library Online Contents | 2007
Aerobic Biodegradation of Polyhydroxybutyrate in Compost
British Library Online Contents | 2011