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Enumerating Salmonella in biosolids for compliance with pathogen regulations
Recently adopted federal regulations (40CFR Part 503) for land use of biosolids set maximum bacterial limits based on fecal coliform or Salmonella and specified test methods to be used. The cited Salmonella methods were not evaluated for their suitability for compliance testing before inclusion in the regulation. This study evaluated the Part 503 Salmonella methods and two other Salmonella methods previously developed to test biosolids and compost. Ten samples each of activated sludge, anaerobically digested biosolids, and compost were analyzed by five different procedures. Statistical analysis (Kruskal‐Wallis one‐way analysis of variance) indicated there was a significant difference between methods (P < 0.001). Pairwise comparisons (Student‐Newman‐Keuls method) showed that the two methods that had been developed for testing biosolids were not significantly (P < 0.05) different and recovered significantly (P < 0.05) more salmonellae than the 503 cited methods. One 503 method failed to detect Salmonella in 43% of samples containing salmonellae. Only one of the three 503 specified Salmonella test methods appeared acceptable for compliance testing.
Enumerating Salmonella in biosolids for compliance with pathogen regulations
Recently adopted federal regulations (40CFR Part 503) for land use of biosolids set maximum bacterial limits based on fecal coliform or Salmonella and specified test methods to be used. The cited Salmonella methods were not evaluated for their suitability for compliance testing before inclusion in the regulation. This study evaluated the Part 503 Salmonella methods and two other Salmonella methods previously developed to test biosolids and compost. Ten samples each of activated sludge, anaerobically digested biosolids, and compost were analyzed by five different procedures. Statistical analysis (Kruskal‐Wallis one‐way analysis of variance) indicated there was a significant difference between methods (P < 0.001). Pairwise comparisons (Student‐Newman‐Keuls method) showed that the two methods that had been developed for testing biosolids were not significantly (P < 0.05) different and recovered significantly (P < 0.05) more salmonellae than the 503 cited methods. One 503 method failed to detect Salmonella in 43% of samples containing salmonellae. Only one of the three 503 specified Salmonella test methods appeared acceptable for compliance testing.
Enumerating Salmonella in biosolids for compliance with pathogen regulations
Yanko, William A. (Autor:in) / Walker, Alan S. (Autor:in) / Jackson, James L. (Autor:in) / Libao, Leticia L. (Autor:in) / Garcia, April L. (Autor:in)
Water Environment Research ; 67 ; 364-370
01.05.1995
7 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
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