Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Single‐Stranded Nucleic Acid Transmembrane Molecular Carriers Based on Positively Charged Helical Foldamers
Transmembrane delivery of biologically active nucleic acids is an important process in cells and has inspired one to develop advanced drug delivery techniques. In this contribution, molecular‐level single‐stranded nucleic acid transmembrane carriers are reported based on 3.2 nm long Huc's foldamers (AOrnQ3Q3)8 and (mQ3Q2)8 with linearly and helically aligned positive charges, respectively. These two foldamers not only show very strong DNA affinity via electrostatic interactions but also discriminatively bind single‐stranded DNA (ss‐DNA) and double‐stranded DNA (ds‐DNA), corroborating the importance of precise charge arrangement in the electrostatic interactions. More importantly, these two foldamers are capable of efficiently transporting ss‐DNA across the lipid membranes, and the ss‐DNA transport activity of (AOrnQ3Q3)8 with linearly aligned charges is higher than that of (mQ3Q2)8 with helically aligned charges. Thus a type of novel single‐stranded nucleic acid transmembrane molecular carriers based on positively charged helical foldamers are introduced. Further, effective and enhanced expression in EGFP‐mRNA transfection experiments strongly demonstrates the potential of positively charged foldamers for RNA transmembrane transport and therapy.
Single‐Stranded Nucleic Acid Transmembrane Molecular Carriers Based on Positively Charged Helical Foldamers
Transmembrane delivery of biologically active nucleic acids is an important process in cells and has inspired one to develop advanced drug delivery techniques. In this contribution, molecular‐level single‐stranded nucleic acid transmembrane carriers are reported based on 3.2 nm long Huc's foldamers (AOrnQ3Q3)8 and (mQ3Q2)8 with linearly and helically aligned positive charges, respectively. These two foldamers not only show very strong DNA affinity via electrostatic interactions but also discriminatively bind single‐stranded DNA (ss‐DNA) and double‐stranded DNA (ds‐DNA), corroborating the importance of precise charge arrangement in the electrostatic interactions. More importantly, these two foldamers are capable of efficiently transporting ss‐DNA across the lipid membranes, and the ss‐DNA transport activity of (AOrnQ3Q3)8 with linearly aligned charges is higher than that of (mQ3Q2)8 with helically aligned charges. Thus a type of novel single‐stranded nucleic acid transmembrane molecular carriers based on positively charged helical foldamers are introduced. Further, effective and enhanced expression in EGFP‐mRNA transfection experiments strongly demonstrates the potential of positively charged foldamers for RNA transmembrane transport and therapy.
Single‐Stranded Nucleic Acid Transmembrane Molecular Carriers Based on Positively Charged Helical Foldamers
Ge, Yunpeng (Autor:in) / Li, Wencan (Autor:in) / Tian, Jun (Autor:in) / Yu, Hao (Autor:in) / Wang, Zhenzhu (Autor:in) / Wang, Ming (Autor:in) / Dong, Zeyuan (Autor:in)
Advanced Science ; 11
01.07.2024
9 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
| Wiley | 2024
Filler compound comprising a positively charged polymer
| Europäisches Patentamt | 2017
Enhanced Osteoconductivity of Positively Charged Titanium Metal
| British Library Online Contents | 2012
Development of algae removal method based on positively charged bubbles
| Online Contents | 2017
Development of algae removal method based on positively charged bubbles
| Springer Verlag | 2017