Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
The Use of a Genetically Engineered Pseudomonas Species (Shk1) as a Bioluminescent Reporter for Heavy Metal Toxicity Screening in Wastewater Treatment Plant Influent
Heavy metals are known to be inhibitory and toxic to the activated‐sludge microbial community in biological wastewater treatment plants. Toxicity screening of aqueous mixtures of these heavy metal ions in plant influent could use both chemical and biological methods. As a biological method, luminescent bacterial bioreporters offer the advantages of a simple test procedure and rapid response. Current biologically based methods for screening aqueous streams for toxicity are labor‐intensive, inaccurate, or difficult to use in continuous monitoring applications. In the present study, a system was developed that is simple and easily automated. This system is based on the bacterium Shk1, a genetically engineered bioluminescent Pseudomonad whose parent strain was originally isolated from activated sludge. Compared with other bioluminescence‐based systems (specifically, the Microtox assay), the system of the present study more accurately reflects the effects of the toxicity of common metal ions on activated‐sludge respirometry without being overly sensitive to typical constituents of wastewater. The use of Shk1 as a bioluminescent reporter for heavy metal toxicity testing for the application of wastewater treatment influent toxicity screening is presented in this study.
The Use of a Genetically Engineered Pseudomonas Species (Shk1) as a Bioluminescent Reporter for Heavy Metal Toxicity Screening in Wastewater Treatment Plant Influent
Heavy metals are known to be inhibitory and toxic to the activated‐sludge microbial community in biological wastewater treatment plants. Toxicity screening of aqueous mixtures of these heavy metal ions in plant influent could use both chemical and biological methods. As a biological method, luminescent bacterial bioreporters offer the advantages of a simple test procedure and rapid response. Current biologically based methods for screening aqueous streams for toxicity are labor‐intensive, inaccurate, or difficult to use in continuous monitoring applications. In the present study, a system was developed that is simple and easily automated. This system is based on the bacterium Shk1, a genetically engineered bioluminescent Pseudomonad whose parent strain was originally isolated from activated sludge. Compared with other bioluminescence‐based systems (specifically, the Microtox assay), the system of the present study more accurately reflects the effects of the toxicity of common metal ions on activated‐sludge respirometry without being overly sensitive to typical constituents of wastewater. The use of Shk1 as a bioluminescent reporter for heavy metal toxicity testing for the application of wastewater treatment influent toxicity screening is presented in this study.
The Use of a Genetically Engineered Pseudomonas Species (Shk1) as a Bioluminescent Reporter for Heavy Metal Toxicity Screening in Wastewater Treatment Plant Influent
Ren, Shijin (Autor:in) / Frymier, Paul D. (Autor:in)
Water Environment Research ; 75 ; 21-29
01.01.2003
9 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
| British Library Online Contents | 2001
Model-Based Construction of Wastewater Treatment Plant Influent Data for Simulation Studies
| DOAJ | 2024