Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Xanthohumol targets the JNK1/2 signaling pathway in apoptosis of human nasopharyngeal carcinoma cells
Nasopharyngeal carcinoma (NPC) is one of the most aggressive malignant tumors of the head and neck. Xanthohumol (Xn) is a compound extracted in a high concentration from the hard resin of hops (Humulus lupulus L.), the basic raw material of beer. This study investigated the apoptotic effect and anticancer properties of Xn in human NPC cell lines. Our study demonstrated that at the concentration 40 μM, Xn significantly reduced cell viability and promoted cell cycle arrest in the G2/M phase in two cell lines. The results indicated that Xn induced apoptosis in NPC cell lines through annexin V/propidium iodide staining, chromatin condensation, and apoptosis‐related pathways. Xn upregulated the expression of apoptosis‐related proteins, namely DR5, cleaved RIP, caspase‐3, caspase‐8, caspase‐9, PARP, Bim, and Bak, and it downregulated the expression of Bcl‐2. Xn upregulated the c‐Jun N‐terminal kinase (JNK) in the mitogen‐activated protein kinase (MAPK), and the inhibition of JNK clearly resulted in decreasing expression of Xn‐activated cleaved caspase‐3 and PARP. Our research provides sufficient evidence to confirm that Xn induces the MAPK JNK pathway to promote apoptosis of NPC and is expected to become a safe and acceptable treatment option for human NPC.
Xanthohumol targets the JNK1/2 signaling pathway in apoptosis of human nasopharyngeal carcinoma cells
Nasopharyngeal carcinoma (NPC) is one of the most aggressive malignant tumors of the head and neck. Xanthohumol (Xn) is a compound extracted in a high concentration from the hard resin of hops (Humulus lupulus L.), the basic raw material of beer. This study investigated the apoptotic effect and anticancer properties of Xn in human NPC cell lines. Our study demonstrated that at the concentration 40 μM, Xn significantly reduced cell viability and promoted cell cycle arrest in the G2/M phase in two cell lines. The results indicated that Xn induced apoptosis in NPC cell lines through annexin V/propidium iodide staining, chromatin condensation, and apoptosis‐related pathways. Xn upregulated the expression of apoptosis‐related proteins, namely DR5, cleaved RIP, caspase‐3, caspase‐8, caspase‐9, PARP, Bim, and Bak, and it downregulated the expression of Bcl‐2. Xn upregulated the c‐Jun N‐terminal kinase (JNK) in the mitogen‐activated protein kinase (MAPK), and the inhibition of JNK clearly resulted in decreasing expression of Xn‐activated cleaved caspase‐3 and PARP. Our research provides sufficient evidence to confirm that Xn induces the MAPK JNK pathway to promote apoptosis of NPC and is expected to become a safe and acceptable treatment option for human NPC.
Xanthohumol targets the JNK1/2 signaling pathway in apoptosis of human nasopharyngeal carcinoma cells
Hsieh, Ming‐Yu (Autor:in) / Hsieh, Ming‐Ju (Autor:in) / Lo, Yu‐Sheng (Autor:in) / Lin, Chia‐Chieh (Autor:in) / Chuang, Yi‐Ching (Autor:in) / Chen, Mu‐Kuan (Autor:in) / Chou, Ming‐Chih (Autor:in)
Environmental Toxicology ; 37 ; 1509-1520
01.06.2022
12 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
Nimbolide induces apoptosis in human nasopharyngeal cancer cells
| Online Contents | 2017