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Comparative evaluation in vitro of the herbicide flurochloridone by cytokinesis‐block micronucleus cytome and comet assays
The in‐vitro effects of flurochloridone and its formulations Twin Pack Gold® (25% a.i.) and Rainbow® (25% a.i.) were evaluated in Chinese Hamster Ovary K1 (CHO‐K1) cells. The cytokinesis‐block micronucleus cytome (CBMN‐cyt) and single‐cell gel electrophoresis (SCGE) assays were used. The activities were tested within the range of final concentrations of 0.25–15 μg flurochloridone/mL. The results demonstrated that both the flurochloridone and Rainbow® were not able to induce micronuclei (MN). On the other hand, Twin Pack Gold® only increased the frequency of MN at 5 μg/mL. Furthermore, 10 and 15 μg/mL of both formulations resulted in a cellular cytotoxicity demonstrated by alterations in the nuclear division index and cellular death. SCGE assay appeared to be a more sensitive bioassay for detecting primary DNA strand breaks at lower concentrations of flurochloridone than MN did. A marked increase in the genetic damage index was observed when 5 and 15 μg/mL of both flurochloridone and Rainbow® but only when 15 μg/mL of Twin Pack Gold® were used. This is the first report demonstrating that flurochloridone and its two commercial formulations are able to induce single‐strand DNA breaks in vitro on mammalian cells. © 2012 Wiley Periodicals, Inc. Environ Toxicol 29: 884–892, 2014.
Comparative evaluation in vitro of the herbicide flurochloridone by cytokinesis‐block micronucleus cytome and comet assays
The in‐vitro effects of flurochloridone and its formulations Twin Pack Gold® (25% a.i.) and Rainbow® (25% a.i.) were evaluated in Chinese Hamster Ovary K1 (CHO‐K1) cells. The cytokinesis‐block micronucleus cytome (CBMN‐cyt) and single‐cell gel electrophoresis (SCGE) assays were used. The activities were tested within the range of final concentrations of 0.25–15 μg flurochloridone/mL. The results demonstrated that both the flurochloridone and Rainbow® were not able to induce micronuclei (MN). On the other hand, Twin Pack Gold® only increased the frequency of MN at 5 μg/mL. Furthermore, 10 and 15 μg/mL of both formulations resulted in a cellular cytotoxicity demonstrated by alterations in the nuclear division index and cellular death. SCGE assay appeared to be a more sensitive bioassay for detecting primary DNA strand breaks at lower concentrations of flurochloridone than MN did. A marked increase in the genetic damage index was observed when 5 and 15 μg/mL of both flurochloridone and Rainbow® but only when 15 μg/mL of Twin Pack Gold® were used. This is the first report demonstrating that flurochloridone and its two commercial formulations are able to induce single‐strand DNA breaks in vitro on mammalian cells. © 2012 Wiley Periodicals, Inc. Environ Toxicol 29: 884–892, 2014.
Comparative evaluation in vitro of the herbicide flurochloridone by cytokinesis‐block micronucleus cytome and comet assays
Nikoloff, Noelia (Autor:in) / Larramendy, Marcelo L. (Autor:in) / Soloneski, Sonia (Autor:in)
Environmental Toxicology ; 29 ; 884-892
01.08.2014
9 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
SYNERGISTIC COMBINATION OF A LENACIL COMPOUND AND FLUROCHLORIDONE FOR DRY FILM PROTECTION
| Europäisches Patentamt | 2016