Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
The effect and mechanism of excessive iodine on the endothelial function of human umbilical vein endothelial cells
Iodine excess (IE) can cause thyroid dysfunction, thyroid diseases can adversely affect cardiovascular function. Accordingly, this study was to explore the direct and indirect effects of IE on endothelial function. Nthy‐ori 3‐1 and HUVECs cells were treated with potassium iodide (KI). CCK‐8, LDH leakage, Elisa, RT‐PCR and Western blotting were used to detect relevant indicators. Results showed that a certain level of KI can directly and indirectly reduce the viability of HUVECs and increase cytotoxicity. KI decreased the expression of ET‐1 and VWF in HUVECs, inhibited the secretion of ET‐1 in culture medium, and increased the expression of IL‐6 and TNFα in HUVECs or Nthy‐ori 3‐1 cells alone. In the co‐culture system, KI decreased the expression of ET‐1 and THBD and increased the expression of TNFα and IL‐6. Collectively, IE can directly and indirectly inhibit endothelial function of endothelial cells, which may be related to its induced inflammatory response.
The effect and mechanism of excessive iodine on the endothelial function of human umbilical vein endothelial cells
Iodine excess (IE) can cause thyroid dysfunction, thyroid diseases can adversely affect cardiovascular function. Accordingly, this study was to explore the direct and indirect effects of IE on endothelial function. Nthy‐ori 3‐1 and HUVECs cells were treated with potassium iodide (KI). CCK‐8, LDH leakage, Elisa, RT‐PCR and Western blotting were used to detect relevant indicators. Results showed that a certain level of KI can directly and indirectly reduce the viability of HUVECs and increase cytotoxicity. KI decreased the expression of ET‐1 and VWF in HUVECs, inhibited the secretion of ET‐1 in culture medium, and increased the expression of IL‐6 and TNFα in HUVECs or Nthy‐ori 3‐1 cells alone. In the co‐culture system, KI decreased the expression of ET‐1 and THBD and increased the expression of TNFα and IL‐6. Collectively, IE can directly and indirectly inhibit endothelial function of endothelial cells, which may be related to its induced inflammatory response.
The effect and mechanism of excessive iodine on the endothelial function of human umbilical vein endothelial cells
Wang, Dandan (Autor:in) / Li, Peng (Autor:in) / Liu, Lixiang (Autor:in) / Liu, Peng (Autor:in) / Zhou, Zheng (Autor:in) / Jin, Meihui (Autor:in) / Li, Baoxiang (Autor:in) / Li, Fan (Autor:in) / Chen, Yao (Autor:in) / Shen, Hongmei (Autor:in)
Environmental Toxicology ; 38 ; 136-145
01.01.2023
10 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
Oxidative modifications of proteins by sodium arsenite in human umbilical vein endothelial cells
| Online Contents | 2011