Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Molecular Characterization of Denitrifying Bacteria Isolated from the Anoxic Reactor of a Modified DEPHANOX Plant Performing Enhanced Biological Phosphorus Removal
Enhanced Biological Phosphorus Removal (EBPR) under anoxic conditions was achieved using a Biological Nutrient Removal (BNR) system based on a modification of the DEPHANOX configuration. Double‐probe Fluorescence in Situ Hybridization (FISH) revealed that Polyphosphate Accumulating Organisms (PAOs) comprised 12.3 ± 3.2% of the total bacterial population in the modified DEPHANOX plant. The growing bacterial population on blood agar and Casitone Glycerol Yeast Autolysate agar (CGYA) medium was 16.7 ± 0.9 × 105 and 3.0 ± 0.6 × 105 colony forming units (cfu) mL−1 activated sludge, respectively. A total of 121 bacterial isolates were characterized according to their denitrification ability, with 26 bacterial strains being capable of reducing nitrate to gas. All denitrifying isolates were placed within the α‐, β‐, and γ‐subdivisions of Proteobacteria and the family Flavobacteriaceae. Furthermore, a novel denitrifying bacterium within the genus Pseudomonas was identified. This is the first report on the isolation and molecular characterization of denitrifying bacteria from EBPR sludge using a DEPHANOX‐type plant.
Molecular Characterization of Denitrifying Bacteria Isolated from the Anoxic Reactor of a Modified DEPHANOX Plant Performing Enhanced Biological Phosphorus Removal
Enhanced Biological Phosphorus Removal (EBPR) under anoxic conditions was achieved using a Biological Nutrient Removal (BNR) system based on a modification of the DEPHANOX configuration. Double‐probe Fluorescence in Situ Hybridization (FISH) revealed that Polyphosphate Accumulating Organisms (PAOs) comprised 12.3 ± 3.2% of the total bacterial population in the modified DEPHANOX plant. The growing bacterial population on blood agar and Casitone Glycerol Yeast Autolysate agar (CGYA) medium was 16.7 ± 0.9 × 105 and 3.0 ± 0.6 × 105 colony forming units (cfu) mL−1 activated sludge, respectively. A total of 121 bacterial isolates were characterized according to their denitrification ability, with 26 bacterial strains being capable of reducing nitrate to gas. All denitrifying isolates were placed within the α‐, β‐, and γ‐subdivisions of Proteobacteria and the family Flavobacteriaceae. Furthermore, a novel denitrifying bacterium within the genus Pseudomonas was identified. This is the first report on the isolation and molecular characterization of denitrifying bacteria from EBPR sludge using a DEPHANOX‐type plant.
Molecular Characterization of Denitrifying Bacteria Isolated from the Anoxic Reactor of a Modified DEPHANOX Plant Performing Enhanced Biological Phosphorus Removal
Zafiriadis, Ilias (Autor:in) / Ntougias, Spyridon (Autor:in) / Mirelis, Paraskevi (Autor:in) / Kapagiannidis, Anastasios G. (Autor:in) / Aivasidis, Alexander (Autor:in)
Water Environment Research ; 84 ; 475-484
01.06.2012
10 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
Biological anoxic phosphorus removal - the DEPHANOX process
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Biological anoxic phosphorus removal - the DEPHANOX process
| British Library Conference Proceedings | 1996
Anoxic phosphorus removal by denitrifying heterotrophic bacteria
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