Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eucalyptol antagonized the apoptosis and immune dysfunction of grass carp hepatocytes induced by tetrabromobisphenol A by regulating ROS/ASK1/JNK pathway
Tetrabromobisphenol A (TBBPA) is a common environmental pollutant which has multi‐organ toxicity to mammals. Eucalyptol (EUC) has super antioxidant biological activity. However, in this experimental study, we probed into the mechanism of toxic of TBBPA exposure on Grass carp hepatocytes (L8824 cells) and the antagonistic impact of EUC on TBBPA. We treated L8824 cells with 8 μg/ml TBBPA and/or 20 μM EUC for 24 h in this test research. The experiment results suggested that TBBPA exposure induced elevated levels of reactive oxygen species (ROS), led to oxidative stress, decreased SOD and CAT activities, decreased GSH and T‐AOC contents, exacerbated MDA accumulation, activated ASK1/JNK signaling pathway, and further increased the contents of mitochondrial dependent apoptosis pathway related indicators (Cyt‐C, Bax, Caspase 9, Caspase 3), while Bcl‐2 expression decreased. In addition, TBBPA exposure induced increased expression of TNF‐α, IL‐6, IL‐1β, and decreased expression of IL‐2, IFN‐γ, Hepcidin, β‐defensin, LEAP2. The oxidative stress level, ASK1/JNK signal pathway expression level, apoptosis ratio and cellular immune function of cells exposed to EUC alone did not change significantly. Combined exposure of TBBPA and EUC significantly reduced the proportion of apoptosis and restored cellular immune function. Therefore, these results suggest that EUC can effectively antagonize TBBPA‐induced apoptosis and immune dysfunction of L8824 cells by regulating ROS/ASK1/JNK signaling pathway.
Eucalyptol antagonized the apoptosis and immune dysfunction of grass carp hepatocytes induced by tetrabromobisphenol A by regulating ROS/ASK1/JNK pathway
Tetrabromobisphenol A (TBBPA) is a common environmental pollutant which has multi‐organ toxicity to mammals. Eucalyptol (EUC) has super antioxidant biological activity. However, in this experimental study, we probed into the mechanism of toxic of TBBPA exposure on Grass carp hepatocytes (L8824 cells) and the antagonistic impact of EUC on TBBPA. We treated L8824 cells with 8 μg/ml TBBPA and/or 20 μM EUC for 24 h in this test research. The experiment results suggested that TBBPA exposure induced elevated levels of reactive oxygen species (ROS), led to oxidative stress, decreased SOD and CAT activities, decreased GSH and T‐AOC contents, exacerbated MDA accumulation, activated ASK1/JNK signaling pathway, and further increased the contents of mitochondrial dependent apoptosis pathway related indicators (Cyt‐C, Bax, Caspase 9, Caspase 3), while Bcl‐2 expression decreased. In addition, TBBPA exposure induced increased expression of TNF‐α, IL‐6, IL‐1β, and decreased expression of IL‐2, IFN‐γ, Hepcidin, β‐defensin, LEAP2. The oxidative stress level, ASK1/JNK signal pathway expression level, apoptosis ratio and cellular immune function of cells exposed to EUC alone did not change significantly. Combined exposure of TBBPA and EUC significantly reduced the proportion of apoptosis and restored cellular immune function. Therefore, these results suggest that EUC can effectively antagonize TBBPA‐induced apoptosis and immune dysfunction of L8824 cells by regulating ROS/ASK1/JNK signaling pathway.
Eucalyptol antagonized the apoptosis and immune dysfunction of grass carp hepatocytes induced by tetrabromobisphenol A by regulating ROS/ASK1/JNK pathway
Sun, Wenying (Autor:in) / Liu, Huanyi (Autor:in) / Zhu, Huijun (Autor:in) / Gao, Meichen (Autor:in) / Xu, Shiwen (Autor:in)
Environmental Toxicology ; 38 ; 820-832
01.03.2023
13 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
Exposure to tetrabromobisphenol A induces cellular dysfunction in osteoblastic MC3T3-E1 cells
| Online Contents | 2017