Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Concentration and Recovery of Coliphages from Water with Bituminous Coal
Coliphages represent a process indicator for fecal pollution. The coal bed concentration method prepared for enterovirus was refined for a concentration of coliphages. A bed made from 1.5 g of 120‐mesh coal powder was used for concentrating coliphage from 200 mL of a water sample with or without the addition of aluminum chloride at different pH values. The isolated E. coli strain EC‐R8 was found to be more susceptible to the desired coliphage and showed significant coliphage–coliform response, with clear plaque used for further studies. The complete coliphage adsorption was achieved with the addition of 0.0005 M AlCl3 at pH 6.0. Adsorbed coliphages were eluted with 3% beef extract in Mcllvaine buffer at pH 7.1, with an average recovery of 78.74%. This concentration technique was applied for the detection of coliphages from the well water of Nagpur city (India) and found to contain coliphages in the range of 2 to 28 plaque‐forming units per liter (PFU/L).
Concentration and Recovery of Coliphages from Water with Bituminous Coal
Coliphages represent a process indicator for fecal pollution. The coal bed concentration method prepared for enterovirus was refined for a concentration of coliphages. A bed made from 1.5 g of 120‐mesh coal powder was used for concentrating coliphage from 200 mL of a water sample with or without the addition of aluminum chloride at different pH values. The isolated E. coli strain EC‐R8 was found to be more susceptible to the desired coliphage and showed significant coliphage–coliform response, with clear plaque used for further studies. The complete coliphage adsorption was achieved with the addition of 0.0005 M AlCl3 at pH 6.0. Adsorbed coliphages were eluted with 3% beef extract in Mcllvaine buffer at pH 7.1, with an average recovery of 78.74%. This concentration technique was applied for the detection of coliphages from the well water of Nagpur city (India) and found to contain coliphages in the range of 2 to 28 plaque‐forming units per liter (PFU/L).
Concentration and Recovery of Coliphages from Water with Bituminous Coal
Dafale, Nishant (Autor:in) / Lakhe, Shrikumar (Autor:in) / Yadav, Krishnakant (Autor:in) / Purohit, Hemant (Autor:in) / Chakrabarti, Tapan (Autor:in)
Water Environment Research ; 80 ; 282-288
01.03.2008
7 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
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