Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Molecular cloning of CYP1A gene and its expression by benzo(a)pyrene from goldfish (Carassius auratus)
10.1002/tox.20422.abs
We cloned and sequenced the cytochrome P450 1A (CYP1A) gene from goldfish (Carassius auratus). It has a 1581 bp open reading frame that encodes a 526 amino acid protein with a theoretical molecular weight of 59.02 kDa. The CYP1A amino acid sequence clusters in a monophyletic group with other fish CYP1As, and more closely related to zebrafish CYP1A (91% identity) than to other fish CYP1As. Exposure to benzo(a)pyrene (BaP) by intraperitoneal injection increased biliary BaP metabolites and liver CYP1A gene expression. BaP exposure also increased CYP1A gene expression in extrahepatic organs, including intestine, and gill, which are sensitive to aqueous and dietary exposure to Arylhydrocarbon receptor (AhR) agonists. Therefore, goldfish CYP1A identified in this study offers basic information for further research related to biomarker use of CYP1A of goldfish. © 2008 Wiley Periodicals, Inc. Environ Toxicol, 2009.
Molecular cloning of CYP1A gene and its expression by benzo(a)pyrene from goldfish (Carassius auratus)
10.1002/tox.20422.abs
We cloned and sequenced the cytochrome P450 1A (CYP1A) gene from goldfish (Carassius auratus). It has a 1581 bp open reading frame that encodes a 526 amino acid protein with a theoretical molecular weight of 59.02 kDa. The CYP1A amino acid sequence clusters in a monophyletic group with other fish CYP1As, and more closely related to zebrafish CYP1A (91% identity) than to other fish CYP1As. Exposure to benzo(a)pyrene (BaP) by intraperitoneal injection increased biliary BaP metabolites and liver CYP1A gene expression. BaP exposure also increased CYP1A gene expression in extrahepatic organs, including intestine, and gill, which are sensitive to aqueous and dietary exposure to Arylhydrocarbon receptor (AhR) agonists. Therefore, goldfish CYP1A identified in this study offers basic information for further research related to biomarker use of CYP1A of goldfish. © 2008 Wiley Periodicals, Inc. Environ Toxicol, 2009.
Molecular cloning of CYP1A gene and its expression by benzo(a)pyrene from goldfish (Carassius auratus)
Oh, Seung‐Min (Autor:in) / Ryu, Byung Taek (Autor:in) / Kim, Ha Ryong (Autor:in) / Choi, Kyunghee (Autor:in) / Chung, Kyu‐Hyuck (Autor:in)
Environmental Toxicology ; 24 ; 225-234
01.06.2009
10 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
Sulfur dioxide and benzo(a)pyrene modulates CYP1A and tumor-related gene expression in rat liver
| Online Contents | 2010
Uptake and accumulation of mercury from dental amalgam in the common goldfish, Carassius auratus
| Online Contents | 2003