Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Employing Response Surface Methodology for Optimization of Mercury Bioremediation by Vibrio parahaemolyticus PG02 in Coastal Sediments of Bushehr, Iran
Three bacterial species of PG01, PG02, and PG03 were isolated from coastal sediments of Bushehr (Iran) as mercury‐resistant bacteria. According to 16S ribosomal DNA gene sequence, the strains were identified as Vibrio natriegens, Vibrio parahaemolyticus, and Oceanimonas baumannii, respectively. Minimum inhibitory concentration test in the presence of HgCl2 revealed that the strain PG02 was the most resistant one, which tolerated up to 45 mg L−1 Hg2+. Tracing the growth curves for the PG02 showed that when it is initially exposed to 5 mg L−1 Hg2+ in the medium, only 10% of its natural growth is reduced, while it simultaneously succeeds to remove 90% of mercury. However, initially 10 mg L−1 Hg2+ in the medium led to 30% growth inhibition and 80% mercury removal. According to a linear relationship between the growth inhibition percentage and the initial mercury concentrations, the median effective concentration was calculated as 21.84 mg L−1 for PG02. Central composite design under response surface methodology well proposed a model with high values of R2 = 0.999 and adjusted R2 = 0.998 which predicted the maximum mercury removal of 89.47% by PG02 under optimum pH of 7.2, temperature of 37.5°C and the initial mercury concentration of 5 mg L−1.
Employing Response Surface Methodology for Optimization of Mercury Bioremediation by Vibrio parahaemolyticus PG02 in Coastal Sediments of Bushehr, Iran
Three bacterial species of PG01, PG02, and PG03 were isolated from coastal sediments of Bushehr (Iran) as mercury‐resistant bacteria. According to 16S ribosomal DNA gene sequence, the strains were identified as Vibrio natriegens, Vibrio parahaemolyticus, and Oceanimonas baumannii, respectively. Minimum inhibitory concentration test in the presence of HgCl2 revealed that the strain PG02 was the most resistant one, which tolerated up to 45 mg L−1 Hg2+. Tracing the growth curves for the PG02 showed that when it is initially exposed to 5 mg L−1 Hg2+ in the medium, only 10% of its natural growth is reduced, while it simultaneously succeeds to remove 90% of mercury. However, initially 10 mg L−1 Hg2+ in the medium led to 30% growth inhibition and 80% mercury removal. According to a linear relationship between the growth inhibition percentage and the initial mercury concentrations, the median effective concentration was calculated as 21.84 mg L−1 for PG02. Central composite design under response surface methodology well proposed a model with high values of R2 = 0.999 and adjusted R2 = 0.998 which predicted the maximum mercury removal of 89.47% by PG02 under optimum pH of 7.2, temperature of 37.5°C and the initial mercury concentration of 5 mg L−1.
Employing Response Surface Methodology for Optimization of Mercury Bioremediation by Vibrio parahaemolyticus PG02 in Coastal Sediments of Bushehr, Iran
Jafari, Seyed Ali (Autor:in) / Cheraghi, Sama (Autor:in) / Mirbakhsh, Maryam (Autor:in) / Mirza, Roozbeh (Autor:in) / Maryamabadi, Ammar (Autor:in)
CLEAN – Soil, Air, Water ; 43 ; 118-126
01.01.2015
9 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
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