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Immobilization of Mycoplana sp. MVMB2 Isolated from Petroleum Contaminated Soil onto Papaya Stem (Carica papaya L.) and Its Application on Degradation of Phenanthrene
This study presents the degradation of phenanthrene using immobilized Mycoplana sp. MVMB2 isolated from contaminated soil. Papaya stem pretreated by two stage processes, treating with acid or alkali and drying, was used for the immobilization of Mycoplana sp. Alkali pretreated papaya stem was found to be most effective in cell uptake compared to acid treated one. The maximum immobilization capacity at various physiochemical conditions for the alkali pretreated papaya stem was found to be at 320 min time, pH 6.5, 30°C temperature, and 18.6 × 106 cells/mL initial concentrations. The adsorption mechanism of Mycoplana sp. MVMB2 on pretreated papaya stem was assessed using various kinetic and isotherm models. The immobilization of Mycoplana sp. MVMB2 on to pretreated papaya stem was corroborated by scanning electron microscopy and Fourier transformed IR spectroscopy analysis. The performance of immobilized cells in batch reactor showed more than 95% phenanthrene degradation within 72 h, whereas, free cells were found to require 120 h. The immobilized cells also showed better degradation performance in the packed column study.
Immobilization of Mycoplana sp. MVMB2 Isolated from Petroleum Contaminated Soil onto Papaya Stem (Carica papaya L.) and Its Application on Degradation of Phenanthrene
This study presents the degradation of phenanthrene using immobilized Mycoplana sp. MVMB2 isolated from contaminated soil. Papaya stem pretreated by two stage processes, treating with acid or alkali and drying, was used for the immobilization of Mycoplana sp. Alkali pretreated papaya stem was found to be most effective in cell uptake compared to acid treated one. The maximum immobilization capacity at various physiochemical conditions for the alkali pretreated papaya stem was found to be at 320 min time, pH 6.5, 30°C temperature, and 18.6 × 106 cells/mL initial concentrations. The adsorption mechanism of Mycoplana sp. MVMB2 on pretreated papaya stem was assessed using various kinetic and isotherm models. The immobilization of Mycoplana sp. MVMB2 on to pretreated papaya stem was corroborated by scanning electron microscopy and Fourier transformed IR spectroscopy analysis. The performance of immobilized cells in batch reactor showed more than 95% phenanthrene degradation within 72 h, whereas, free cells were found to require 120 h. The immobilized cells also showed better degradation performance in the packed column study.
Immobilization of Mycoplana sp. MVMB2 Isolated from Petroleum Contaminated Soil onto Papaya Stem (Carica papaya L.) and Its Application on Degradation of Phenanthrene
Brinda Lakshmi, Mahalingam (Autor:in) / Muthukumar, Karuppan (Autor:in) / Velan, Manickam (Autor:in)
CLEAN – Soil, Air, Water ; 40 ; 870-877
01.08.2012
8 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
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