Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Eine Plattform für die Wissenschaft: Bauingenieurwesen, Architektur und Urbanistik
Effect of microcystin‐LR on human placental villous trophoblast differentiation in vitro
Microcystin‐LR is a cyanobacterial toxin found in surface and recreational waters that inhibits protein phosphatases and may disrupt the cytoskeleton. Microcystins induce apoptosis in hepatocytes at ≤2.0 µM. Nothing is known about the effects of microcystins on human placental trophoblast differentiation and function. The differentiation of villous trophoblasts to form syncytiotrophoblast occurs throughout pregnancy and is essential for normal placental and fetal development. To investigate the effects of microcystin, villous cytotrophoblasts were isolated from term placentas using an established method and exposed to microcystin‐LR. Microcystin‐LR below the cytotoxic dose of 25 µM did not cause cell rounding or detachment, had no effect on apoptosis, and no effect on the morphological differentiation of mononucleated cytotrophoblasts to multinucleated syncytiotrophoblast. However, secretion of human chorionic gonadotropin (hCG) increased in a microcystin‐LR dose‐dependent manner. When incubated with l‐buthionine sulphoximine (BSO) to deplete glutathione levels, trophoblast morphological differentiation proceeded normally in the presence of microcystin‐LR. Microcystin‐LR did not disrupt the trophoblast microtubule cytoskeleton, which is known to play a role in trophoblast differentiation. Immunofluorescence studies showed that trophoblasts express organic anion transport protein 1B3 (OATP1B3), a known microcystin transport protein. In comparison to hepatocytes, trophoblasts appear to be more resistant to the toxic effects of microcystin‐LR. The physiological implications of increased hCG secretion in response to microcystin‐LR exposure remain to be determined. © 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 427–439, 2016.
Effect of microcystin‐LR on human placental villous trophoblast differentiation in vitro
Microcystin‐LR is a cyanobacterial toxin found in surface and recreational waters that inhibits protein phosphatases and may disrupt the cytoskeleton. Microcystins induce apoptosis in hepatocytes at ≤2.0 µM. Nothing is known about the effects of microcystins on human placental trophoblast differentiation and function. The differentiation of villous trophoblasts to form syncytiotrophoblast occurs throughout pregnancy and is essential for normal placental and fetal development. To investigate the effects of microcystin, villous cytotrophoblasts were isolated from term placentas using an established method and exposed to microcystin‐LR. Microcystin‐LR below the cytotoxic dose of 25 µM did not cause cell rounding or detachment, had no effect on apoptosis, and no effect on the morphological differentiation of mononucleated cytotrophoblasts to multinucleated syncytiotrophoblast. However, secretion of human chorionic gonadotropin (hCG) increased in a microcystin‐LR dose‐dependent manner. When incubated with l‐buthionine sulphoximine (BSO) to deplete glutathione levels, trophoblast morphological differentiation proceeded normally in the presence of microcystin‐LR. Microcystin‐LR did not disrupt the trophoblast microtubule cytoskeleton, which is known to play a role in trophoblast differentiation. Immunofluorescence studies showed that trophoblasts express organic anion transport protein 1B3 (OATP1B3), a known microcystin transport protein. In comparison to hepatocytes, trophoblasts appear to be more resistant to the toxic effects of microcystin‐LR. The physiological implications of increased hCG secretion in response to microcystin‐LR exposure remain to be determined. © 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 427–439, 2016.
Effect of microcystin‐LR on human placental villous trophoblast differentiation in vitro
Douglas, Gordon C. (Autor:in) / Thirkill, Twanda L. (Autor:in) / Kumar, Priyadarsini (Autor:in) / Loi, Minerva (Autor:in) / Hilborn, Elizabeth D. (Autor:in)
Environmental Toxicology ; 31 ; 427-439
01.04.2016
13 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
Effect of microcystin‐LR on human placental villous trophoblast differentiation in vitro
| Online Contents | 2016
Serologic evaluation of human microcystin exposure
| Wiley | 2007
How microcystin‐degrading bacteria express microcystin degradation activity
| Online Contents | 2011