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Measuring Airborne Antibiotic Resistance Genes in Swiss Cities via a DNA-Enabled Electrochemical Chip-Based Sensor
https://orcid.org/0000-0003-2078-137X
Antibiotic resistance genes (ARGs) as an emerging airborne pollutants are a significant and growing concern for public and environmental health. Accurate surveillance and quantitation of low-dose ARGs in the air have become critical in providing evidence-based public risk assessment for population health management. With this work, we introduced a portable and sensitive electrochemical (EC) bioanalytical platform for detecting airborne ARGs, which relied on the EC current response of surface-initiated ARGs hybridization. These low-cost chips for EC ARGs sensing exhibited good thermal (25∼60 °C) and long-term stability (6 days). The prototype of the biosensor system demonstrated an ultralow limit of detection (7.4 fM) and excellent anti-interference performance. For the first time, an EC sensor was applied successfully to identify and quantitate two types of ARGs (floR and bla-TEM genes) from real-world PM2.5 (particulate matter with aerodynamic diameters less than 2.5 μm) samples. Results for PM2.5 collected from Bern (urban area), Basel (suburban area), Zürich (urban area), Sion (airport highway area), and Rigi (rural and high-altitude area) in Switzerland were obtained, in favorable agreement with gel electrophoresis analysis. The results indicated that our EC biosensor offered a reliable and attractive alternative to the current methods for airborne ARG detection.
Measuring Airborne Antibiotic Resistance Genes in Swiss Cities via a DNA-Enabled Electrochemical Chip-Based Sensor
https://orcid.org/0000-0003-2078-137X
Antibiotic resistance genes (ARGs) as an emerging airborne pollutants are a significant and growing concern for public and environmental health. Accurate surveillance and quantitation of low-dose ARGs in the air have become critical in providing evidence-based public risk assessment for population health management. With this work, we introduced a portable and sensitive electrochemical (EC) bioanalytical platform for detecting airborne ARGs, which relied on the EC current response of surface-initiated ARGs hybridization. These low-cost chips for EC ARGs sensing exhibited good thermal (25∼60 °C) and long-term stability (6 days). The prototype of the biosensor system demonstrated an ultralow limit of detection (7.4 fM) and excellent anti-interference performance. For the first time, an EC sensor was applied successfully to identify and quantitate two types of ARGs (floR and bla-TEM genes) from real-world PM2.5 (particulate matter with aerodynamic diameters less than 2.5 μm) samples. Results for PM2.5 collected from Bern (urban area), Basel (suburban area), Zürich (urban area), Sion (airport highway area), and Rigi (rural and high-altitude area) in Switzerland were obtained, in favorable agreement with gel electrophoresis analysis. The results indicated that our EC biosensor offered a reliable and attractive alternative to the current methods for airborne ARG detection.
Measuring Airborne Antibiotic Resistance Genes in Swiss Cities via a DNA-Enabled Electrochemical Chip-Based Sensor
https://orcid.org/0000-0003-2078-137X
Antibiotic resistance genes (ARGs) as an emerging airborne pollutants are a significant and growing concern for public and environmental health. Accurate surveillance and quantitation of low-dose ARGs in the air have become critical in providing evidence-based public risk assessment for population health management. With this work, we introduced a portable and sensitive electrochemical (EC) bioanalytical platform for detecting airborne ARGs, which relied on the EC current response of surface-initiated ARGs hybridization. These low-cost chips for EC ARGs sensing exhibited good thermal (25∼60 °C) and long-term stability (6 days). The prototype of the biosensor system demonstrated an ultralow limit of detection (7.4 fM) and excellent anti-interference performance. For the first time, an EC sensor was applied successfully to identify and quantitate two types of ARGs (floR and bla-TEM genes) from real-world PM2.5 (particulate matter with aerodynamic diameters less than 2.5 μm) samples. Results for PM2.5 collected from Bern (urban area), Basel (suburban area), Zürich (urban area), Sion (airport highway area), and Rigi (rural and high-altitude area) in Switzerland were obtained, in favorable agreement with gel electrophoresis analysis. The results indicated that our EC biosensor offered a reliable and attractive alternative to the current methods for airborne ARG detection.
Measuring Airborne Antibiotic Resistance Genes in Swiss Cities via a DNA-Enabled Electrochemical Chip-Based Sensor
Liu, Bei (author) / Jiang, Fuze (author) / Tao, Yile (author) / Zheng, Tong (author) / Yue, Yang (author) / Tepper, Viktoria (author) / Cao, Xiaobao (author) / Wang, Jing (author)
ACS ES&T Engineering ; 2 ; 1677-1683
2022-09-09
Article (Journal)
Electronic Resource
English
| American Chemical Society | 2023