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Confronting Assumptions of Phosphorus-Accumulating Organisms and Glycogen-Accumulating Organisms: Peaceful Coexistence in a Carbon-Limited Sidestream EBPR Demonstration
https://orcid.org/0000-0001-8795-0479
https://orcid.org/0000-0002-6369-9432
https://orcid.org/0000-0002-7016-1307
https://orcid.org/0000-0001-7516-1085
https://orcid.org/0000-0002-9100-1628
Enhanced biological phosphorus removal (EBPR) is strongly influenced by the influent ratio of readily biodegradable carbon to soluble phosphorus due to the preferences of phosphorus-accumulating organisms (PAO). The sidestream EBPR (S2EBPR) process redirects a portion of return activated sludge (RAS) to a sidestream fermenter, increasing the availability of biodegradable carbon. In this study, we assessed the performance and microbial community structure of a full-scale S2EBPR demonstration supplemented with external carbon dosing. By the end of the 10 month study period, the demonstration achieved a median effluent orthophosphate of 0.3 mg/L. The microbial community consisted of a common core microbiome in the RAS fermenter, EBPR basin, and nitrification basin. The most abundant PAO detected were Ca. “Dechloromonas phosphorivorans”, while the canonical PAO Ca. “Accumulibacter” and Tetrasphaera were observed in lower relative abundance. In addition to non-canonical PAO enrichment, the glycogen-accumulating organism Ca. “Competibacter” proliferated throughout the study and at some points outnumbered PAO taxa by 30 to 1 with no tangible performance impacts. This study provides insights into successful S2EBPR implementation at a low-carbon facility and improves our understanding of microbial community structure and key PAO and GAO in S2EBPR systems.
Phosphorus-accumulating organism Dechloromonas and glycogen-accumulating organism Ca. Competibacter coexisted in a full-scale sidestream-enhanced biological phosphorus removal demonstration.
Confronting Assumptions of Phosphorus-Accumulating Organisms and Glycogen-Accumulating Organisms: Peaceful Coexistence in a Carbon-Limited Sidestream EBPR Demonstration
https://orcid.org/0000-0001-8795-0479
https://orcid.org/0000-0002-6369-9432
https://orcid.org/0000-0002-7016-1307
https://orcid.org/0000-0001-7516-1085
https://orcid.org/0000-0002-9100-1628
Enhanced biological phosphorus removal (EBPR) is strongly influenced by the influent ratio of readily biodegradable carbon to soluble phosphorus due to the preferences of phosphorus-accumulating organisms (PAO). The sidestream EBPR (S2EBPR) process redirects a portion of return activated sludge (RAS) to a sidestream fermenter, increasing the availability of biodegradable carbon. In this study, we assessed the performance and microbial community structure of a full-scale S2EBPR demonstration supplemented with external carbon dosing. By the end of the 10 month study period, the demonstration achieved a median effluent orthophosphate of 0.3 mg/L. The microbial community consisted of a common core microbiome in the RAS fermenter, EBPR basin, and nitrification basin. The most abundant PAO detected were Ca. “Dechloromonas phosphorivorans”, while the canonical PAO Ca. “Accumulibacter” and Tetrasphaera were observed in lower relative abundance. In addition to non-canonical PAO enrichment, the glycogen-accumulating organism Ca. “Competibacter” proliferated throughout the study and at some points outnumbered PAO taxa by 30 to 1 with no tangible performance impacts. This study provides insights into successful S2EBPR implementation at a low-carbon facility and improves our understanding of microbial community structure and key PAO and GAO in S2EBPR systems.
Phosphorus-accumulating organism Dechloromonas and glycogen-accumulating organism Ca. Competibacter coexisted in a full-scale sidestream-enhanced biological phosphorus removal demonstration.
Confronting Assumptions of Phosphorus-Accumulating Organisms and Glycogen-Accumulating Organisms: Peaceful Coexistence in a Carbon-Limited Sidestream EBPR Demonstration
https://orcid.org/0000-0001-8795-0479
https://orcid.org/0000-0002-6369-9432
https://orcid.org/0000-0002-7016-1307
https://orcid.org/0000-0001-7516-1085
https://orcid.org/0000-0002-9100-1628
Enhanced biological phosphorus removal (EBPR) is strongly influenced by the influent ratio of readily biodegradable carbon to soluble phosphorus due to the preferences of phosphorus-accumulating organisms (PAO). The sidestream EBPR (S2EBPR) process redirects a portion of return activated sludge (RAS) to a sidestream fermenter, increasing the availability of biodegradable carbon. In this study, we assessed the performance and microbial community structure of a full-scale S2EBPR demonstration supplemented with external carbon dosing. By the end of the 10 month study period, the demonstration achieved a median effluent orthophosphate of 0.3 mg/L. The microbial community consisted of a common core microbiome in the RAS fermenter, EBPR basin, and nitrification basin. The most abundant PAO detected were Ca. “Dechloromonas phosphorivorans”, while the canonical PAO Ca. “Accumulibacter” and Tetrasphaera were observed in lower relative abundance. In addition to non-canonical PAO enrichment, the glycogen-accumulating organism Ca. “Competibacter” proliferated throughout the study and at some points outnumbered PAO taxa by 30 to 1 with no tangible performance impacts. This study provides insights into successful S2EBPR implementation at a low-carbon facility and improves our understanding of microbial community structure and key PAO and GAO in S2EBPR systems.
Phosphorus-accumulating organism Dechloromonas and glycogen-accumulating organism Ca. Competibacter coexisted in a full-scale sidestream-enhanced biological phosphorus removal demonstration.
Confronting Assumptions of Phosphorus-Accumulating Organisms and Glycogen-Accumulating Organisms: Peaceful Coexistence in a Carbon-Limited Sidestream EBPR Demonstration
Farmer, McKenna (author) / Sabba, Fabrizio (author) / Jia, Zhen (author) / Dunlap, Patrick (author) / Barnard, James (author) / Qin, Cindy Dongqi (author) / Straka, Levi (author) / Kozak, Joseph A. (author) / Downing, Leon (author) / Wells, George (author)
ACS ES&T Water ; 3 ; 2384-2394
2023-08-11
Article (Journal)
Electronic Resource
English
S2EBPR , phosphorus , PAO , GAO , RAS fermentation
Why Phosphate Accumulating Organisms (PAOs) Win the Competition in EBPR Systems
| British Library Conference Proceedings | 2004