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Pharmaceutical Attenuation Differs within Woodchip-Based Lignocellulose Bioreactors across Nitrate- and Sulfate-Reducing Conditions
https://orcid.org/0000-0001-8370-4991
https://orcid.org/0000-0002-7034-2431
https://orcid.org/0000-0002-5870-1569
https://orcid.org/0000-0003-1686-8464
https://orcid.org/0000-0002-2942-1066
Lignocellulosic bioreactors use solid-phase substrates such as woodchips to sustain microbial respiration and have been applied to treat agricultural runoff, stormwater, and other impaired waters. Here, we query how respiration of different soluble electron acceptors impacts the degradation of environmentally relevant pharmaceuticals associated with treated municipal wastewater discharge. Laboratory-scale columns containing a mixture of woodchips and alfalfa were manipulated across nitrate- and sulfate-reducing conditions using residence time and influent composition. Under steady-state conditions, bioreactors dominated by nitrate reduction harbored a distinct phylogenetic profile containing the genera Denitratisoma and increases in the denitrification gene nirS. In contrast, bioreactors where sulfate reduction dominated exhibited increased relative abundance of fermenters (e.g., Obscuribacteriales) and putative sulfate reducers (e.g., Desulfobulbus). Atenolol attenuation and biotransformation to carboxy-metoprolol accelerated under nitrate-reducing conditions; in contrast, trimethoprim attenuation and biotransformation to desmethyl trimethoprim was nearly an order of magnitude faster under sulfate-reducing conditions. Modest sulfamethoxazole attenuation occurred under all tested conditions. Denitrification-associated rate constants for atenolol were comparable to those reported in constructed wetlands and aquifer recharge, suggesting commonality in biotransformation mechanisms. Collectively, results suggest that manipulation of biogeochemical gradients during nature-based treatment can be applied to attenuate nitrate and trace quantities of pharmaceuticals.
Biologically mediated attenuation of atenolol is faster during nitrate reduction, whereas trimethoprim rates accelerate during sulfate reduction within woodchip/lignocellulose bioreactors. Sulfamethoxazole attenuation rates were constant across these conditions.
Pharmaceutical Attenuation Differs within Woodchip-Based Lignocellulose Bioreactors across Nitrate- and Sulfate-Reducing Conditions
https://orcid.org/0000-0001-8370-4991
https://orcid.org/0000-0002-7034-2431
https://orcid.org/0000-0002-5870-1569
https://orcid.org/0000-0003-1686-8464
https://orcid.org/0000-0002-2942-1066
Lignocellulosic bioreactors use solid-phase substrates such as woodchips to sustain microbial respiration and have been applied to treat agricultural runoff, stormwater, and other impaired waters. Here, we query how respiration of different soluble electron acceptors impacts the degradation of environmentally relevant pharmaceuticals associated with treated municipal wastewater discharge. Laboratory-scale columns containing a mixture of woodchips and alfalfa were manipulated across nitrate- and sulfate-reducing conditions using residence time and influent composition. Under steady-state conditions, bioreactors dominated by nitrate reduction harbored a distinct phylogenetic profile containing the genera Denitratisoma and increases in the denitrification gene nirS. In contrast, bioreactors where sulfate reduction dominated exhibited increased relative abundance of fermenters (e.g., Obscuribacteriales) and putative sulfate reducers (e.g., Desulfobulbus). Atenolol attenuation and biotransformation to carboxy-metoprolol accelerated under nitrate-reducing conditions; in contrast, trimethoprim attenuation and biotransformation to desmethyl trimethoprim was nearly an order of magnitude faster under sulfate-reducing conditions. Modest sulfamethoxazole attenuation occurred under all tested conditions. Denitrification-associated rate constants for atenolol were comparable to those reported in constructed wetlands and aquifer recharge, suggesting commonality in biotransformation mechanisms. Collectively, results suggest that manipulation of biogeochemical gradients during nature-based treatment can be applied to attenuate nitrate and trace quantities of pharmaceuticals.
Biologically mediated attenuation of atenolol is faster during nitrate reduction, whereas trimethoprim rates accelerate during sulfate reduction within woodchip/lignocellulose bioreactors. Sulfamethoxazole attenuation rates were constant across these conditions.
Pharmaceutical Attenuation Differs within Woodchip-Based Lignocellulose Bioreactors across Nitrate- and Sulfate-Reducing Conditions
https://orcid.org/0000-0001-8370-4991
https://orcid.org/0000-0002-7034-2431
https://orcid.org/0000-0002-5870-1569
https://orcid.org/0000-0003-1686-8464
https://orcid.org/0000-0002-2942-1066
Lignocellulosic bioreactors use solid-phase substrates such as woodchips to sustain microbial respiration and have been applied to treat agricultural runoff, stormwater, and other impaired waters. Here, we query how respiration of different soluble electron acceptors impacts the degradation of environmentally relevant pharmaceuticals associated with treated municipal wastewater discharge. Laboratory-scale columns containing a mixture of woodchips and alfalfa were manipulated across nitrate- and sulfate-reducing conditions using residence time and influent composition. Under steady-state conditions, bioreactors dominated by nitrate reduction harbored a distinct phylogenetic profile containing the genera Denitratisoma and increases in the denitrification gene nirS. In contrast, bioreactors where sulfate reduction dominated exhibited increased relative abundance of fermenters (e.g., Obscuribacteriales) and putative sulfate reducers (e.g., Desulfobulbus). Atenolol attenuation and biotransformation to carboxy-metoprolol accelerated under nitrate-reducing conditions; in contrast, trimethoprim attenuation and biotransformation to desmethyl trimethoprim was nearly an order of magnitude faster under sulfate-reducing conditions. Modest sulfamethoxazole attenuation occurred under all tested conditions. Denitrification-associated rate constants for atenolol were comparable to those reported in constructed wetlands and aquifer recharge, suggesting commonality in biotransformation mechanisms. Collectively, results suggest that manipulation of biogeochemical gradients during nature-based treatment can be applied to attenuate nitrate and trace quantities of pharmaceuticals.
Biologically mediated attenuation of atenolol is faster during nitrate reduction, whereas trimethoprim rates accelerate during sulfate reduction within woodchip/lignocellulose bioreactors. Sulfamethoxazole attenuation rates were constant across these conditions.
Pharmaceutical Attenuation Differs within Woodchip-Based Lignocellulose Bioreactors across Nitrate- and Sulfate-Reducing Conditions
Brady, Adam R. (author) / Vega, Michael A. P. (author) / Stiegler, Angela N. (author) / Scholes, Rachel C. (author) / Riddle, Kimberly N. (author) / Peel, Henry F. (author) / Sedlak, David L. (author) / Sharp, Jonathan O. (author)
ACS ES&T Water ; 3 ; 1352-1363
2023-05-12
Article (Journal)
Electronic Resource
English
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