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Dual Role of Cu2+ in the Physiological Growth and Metabolism of Microcystis aeruginosa
https://orcid.org/0000-0001-7722-5126
https://orcid.org/0000-0002-4960-3000
https://orcid.org/0000-0003-2990-7311
https://orcid.org/0000-0002-1550-4258
https://orcid.org/0000-0003-3460-2491
Cyanobacterial bloom is prevalent in freshwater of China and usually controlled by CuSO4. However, the remaining available Cu2+ effects on cyanobacterial growth and metabolism have not been fully investigated. Therefore, we here investigated different CuSO4 concentrations on the growth and metabolism of typical toxic cyanobacteria, Microcystis aeruginosa. The results showed that low CuSO4 concentrations (<0.1 mg/L) facilitate algal growth, photosynthesis, and enzyme activity activities. Conversely, high CuSO4 concentrations (>0.1 mg/L) inhibited the growth and markedly reduced the photosynthesis of oxidative enzymes. Notably, the algae secreted more extracellular polysaccharides (EPS) and released microcystin to mitigate the high Cu2+ stress compared to low concentrations. Moreover, the results illustrated how Cu2+ influenced the expression of key genes on the mRNA level. Specifically, capD (coded for polysaccharides), mcy (microcystin), and furA (mcy promoter) genes, were upregulated in high CuSO4 concentrations, while the photosynthetic rbcL gene was downregulated. The furA regulated the mcy gene cluster promoter, which indirectly mediated the microcystin release. The upregulation of the complex genes revealed the intricate genetic responses of M. aeruginosa to Cu2+ stress. This study explored the dual roles of CuSO4 in algae growth and molecular mechanisms, providing a new perspective on understanding the risks of algaecides application.
A new perspective on algaecide application in freshwater: dual role of Cu2+ in the growth and metabolism of Microcystis aeruginosa
Dual Role of Cu2+ in the Physiological Growth and Metabolism of Microcystis aeruginosa
https://orcid.org/0000-0001-7722-5126
https://orcid.org/0000-0002-4960-3000
https://orcid.org/0000-0003-2990-7311
https://orcid.org/0000-0002-1550-4258
https://orcid.org/0000-0003-3460-2491
Cyanobacterial bloom is prevalent in freshwater of China and usually controlled by CuSO4. However, the remaining available Cu2+ effects on cyanobacterial growth and metabolism have not been fully investigated. Therefore, we here investigated different CuSO4 concentrations on the growth and metabolism of typical toxic cyanobacteria, Microcystis aeruginosa. The results showed that low CuSO4 concentrations (<0.1 mg/L) facilitate algal growth, photosynthesis, and enzyme activity activities. Conversely, high CuSO4 concentrations (>0.1 mg/L) inhibited the growth and markedly reduced the photosynthesis of oxidative enzymes. Notably, the algae secreted more extracellular polysaccharides (EPS) and released microcystin to mitigate the high Cu2+ stress compared to low concentrations. Moreover, the results illustrated how Cu2+ influenced the expression of key genes on the mRNA level. Specifically, capD (coded for polysaccharides), mcy (microcystin), and furA (mcy promoter) genes, were upregulated in high CuSO4 concentrations, while the photosynthetic rbcL gene was downregulated. The furA regulated the mcy gene cluster promoter, which indirectly mediated the microcystin release. The upregulation of the complex genes revealed the intricate genetic responses of M. aeruginosa to Cu2+ stress. This study explored the dual roles of CuSO4 in algae growth and molecular mechanisms, providing a new perspective on understanding the risks of algaecides application.
A new perspective on algaecide application in freshwater: dual role of Cu2+ in the growth and metabolism of Microcystis aeruginosa
Dual Role of Cu2+ in the Physiological Growth and Metabolism of Microcystis aeruginosa
https://orcid.org/0000-0001-7722-5126
https://orcid.org/0000-0002-4960-3000
https://orcid.org/0000-0003-2990-7311
https://orcid.org/0000-0002-1550-4258
https://orcid.org/0000-0003-3460-2491
Cyanobacterial bloom is prevalent in freshwater of China and usually controlled by CuSO4. However, the remaining available Cu2+ effects on cyanobacterial growth and metabolism have not been fully investigated. Therefore, we here investigated different CuSO4 concentrations on the growth and metabolism of typical toxic cyanobacteria, Microcystis aeruginosa. The results showed that low CuSO4 concentrations (<0.1 mg/L) facilitate algal growth, photosynthesis, and enzyme activity activities. Conversely, high CuSO4 concentrations (>0.1 mg/L) inhibited the growth and markedly reduced the photosynthesis of oxidative enzymes. Notably, the algae secreted more extracellular polysaccharides (EPS) and released microcystin to mitigate the high Cu2+ stress compared to low concentrations. Moreover, the results illustrated how Cu2+ influenced the expression of key genes on the mRNA level. Specifically, capD (coded for polysaccharides), mcy (microcystin), and furA (mcy promoter) genes, were upregulated in high CuSO4 concentrations, while the photosynthetic rbcL gene was downregulated. The furA regulated the mcy gene cluster promoter, which indirectly mediated the microcystin release. The upregulation of the complex genes revealed the intricate genetic responses of M. aeruginosa to Cu2+ stress. This study explored the dual roles of CuSO4 in algae growth and molecular mechanisms, providing a new perspective on understanding the risks of algaecides application.
A new perspective on algaecide application in freshwater: dual role of Cu2+ in the growth and metabolism of Microcystis aeruginosa
Dual Role of Cu2+ in the Physiological Growth and Metabolism of Microcystis aeruginosa
Zhang, Lingping (author) / Wu, Yunjia (author) / Lei, Pei (author) / Xie, Wen-Ming (author) / Liu, Jin-e (author) / Meng, Han (author) / Li, Shiyin (author) / He, Huan (author) / Wang, Guoxiang (author) / Zhang, Limin (author)
ACS ES&T Water ; 4 ; 4636-4645
2024-10-11
Article (Journal)
Electronic Resource
English
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