A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Stenotrophomonas maltophilia in cystic fibrosis ; Stenotrophomonas maltophilia in Mukoviszidose
Cystic fibrosis (CF) is an autosomal recessive disorder that affects mainly the Caucasian population. CF patients frequently suffer from chronic bacterial airway infections, which are progressively described as an important cause of high morbidity and mortality rates among these individuals. In this scenario, Stenotrophomonas maltophilia, an environmental Gram-negative rod, has been lately reported as a global emerging bacterium by CF centres worldwide. At the moment, it is still unclear whether S. maltophilia is causally related to disease progression or if this pathogen is real a marker of the CF disease’s severity. In the present study, the relevance and importance of S. maltophilia was investigated. The major findings were: The use of selective medium for S. maltophilia supported the growth of the S. maltophilia 64.0 % more than the conventional media. It was also verified that tigecycline, trimethoprim-sulfamethoxazole, fosfomycin and levofloxacin exhibited good in vitro activity against S. maltophilia, while ceftazidime, colistin and ticarcillin-clavulanate acid had low activity against this bacterium. Different fingerprinting methods, Enterobacterial Repetitive Intergenic Consensus - polymerase chain reaction (ERIC-PCR) and semi-automated Repetitive Sequence-Based-polymerase chain reaction (rep-PCR) DiversiLab® system, demonstrated high degree of genetic heterogeneity among both clinical (CF and ICU) and environmental S. maltophilia isolates. Further, gas chromatography data revealed that S. maltophilia samples from CF individuals produced a significant higher amount of fatty acids in comparison to samples obtained from ICU patients and environment. Interestingly, principal component analysis based on Matrix-Assisted Laser Desorption Ionisation-Time of Flight Mass Spectrometry (MALDI-TOF MS) spectra revealed that S. maltophilia isolates from CF patients are clustered together in a separated group, whereas ICU and environmental samples grouped in a mixed manner indicating adaptation or strains that ...
Stenotrophomonas maltophilia in cystic fibrosis ; Stenotrophomonas maltophilia in Mukoviszidose
Cystic fibrosis (CF) is an autosomal recessive disorder that affects mainly the Caucasian population. CF patients frequently suffer from chronic bacterial airway infections, which are progressively described as an important cause of high morbidity and mortality rates among these individuals. In this scenario, Stenotrophomonas maltophilia, an environmental Gram-negative rod, has been lately reported as a global emerging bacterium by CF centres worldwide. At the moment, it is still unclear whether S. maltophilia is causally related to disease progression or if this pathogen is real a marker of the CF disease’s severity. In the present study, the relevance and importance of S. maltophilia was investigated. The major findings were: The use of selective medium for S. maltophilia supported the growth of the S. maltophilia 64.0 % more than the conventional media. It was also verified that tigecycline, trimethoprim-sulfamethoxazole, fosfomycin and levofloxacin exhibited good in vitro activity against S. maltophilia, while ceftazidime, colistin and ticarcillin-clavulanate acid had low activity against this bacterium. Different fingerprinting methods, Enterobacterial Repetitive Intergenic Consensus - polymerase chain reaction (ERIC-PCR) and semi-automated Repetitive Sequence-Based-polymerase chain reaction (rep-PCR) DiversiLab® system, demonstrated high degree of genetic heterogeneity among both clinical (CF and ICU) and environmental S. maltophilia isolates. Further, gas chromatography data revealed that S. maltophilia samples from CF individuals produced a significant higher amount of fatty acids in comparison to samples obtained from ICU patients and environment. Interestingly, principal component analysis based on Matrix-Assisted Laser Desorption Ionisation-Time of Flight Mass Spectrometry (MALDI-TOF MS) spectra revealed that S. maltophilia isolates from CF patients are clustered together in a separated group, whereas ICU and environmental samples grouped in a mixed manner indicating adaptation or strains that ...
Stenotrophomonas maltophilia in cystic fibrosis ; Stenotrophomonas maltophilia in Mukoviszidose
Goncalves Vidigal, Pedrina (author)
2014-09-08
Theses
Electronic Resource
English
| Taylor & Francis Verlag | 2002