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On the search for the vernalization locus in caraway (Carum carvi) using genotyping by sequencing data ; Auf der Suche nach dem Vernalisations-Locus in Kümmel (Carum carvi) mittels Genotyping by Sequencing
Caraway (Carum carvi) can be divided into biennial and annual flowering types. Biennials require a cold stimulus for initiation of flowering, whereas annuals lack any vernalization requirement. Cultivation of annuals is considered advantageous due to better integration into crop rotation and reduced production costs. However, available annual varieties lack a sufficient yield potential and essential oil content. Valuable genetic diversity exists within the biennial genepool, which can be transferred to the annual genepool by crossbreeding. According to literature, F1 plants from crosses between annual and biennial flowering types uniformly show annual flowering. Resulting F2 populations show a Mendelian 3:1 ratio between annual and biennial flowering types. This indicates a monogenic inheritance of vernalization requirement in caraway with dominant annual flowering. After crossbreeding of annual and biennial flowering types, the recessive allele for biennial flowering can remain within the breeding material over multiple generations. Thus, a genetic marker associated with the vernalization locus would be beneficial to detect the recessive allele in one selection step. To identify markers associated with vernalization requirement, we used genotyping by sequencing (GBS) data of 70 biennial and 67 annual flowering types. We conducted case-control studies and population differentiation-based tests. In total, 60 common single nucleotide polymorphism (SNP) markers were found that were significantly associated with vernalization requirement. Out of these, we developed 19 diagnostic markers. In future, these diagnostic markers might be tested in segregating populations. ; Kümmel (Carum carvi) kann in zweijährige und einjährige Blühtypen unterteilt werden. Zweijährige benötigen einen Kältereiz für die Blühinduktion, während Einjährige keinen Vernalisationsbedarf haben. Aufgrund der besseren Integration in die Fruchtfolge und der geringeren Produktionskosten wird der Anbau Einjähriger als vorteilhaft angesehen. Die ...
On the search for the vernalization locus in caraway (Carum carvi) using genotyping by sequencing data ; Auf der Suche nach dem Vernalisations-Locus in Kümmel (Carum carvi) mittels Genotyping by Sequencing
Caraway (Carum carvi) can be divided into biennial and annual flowering types. Biennials require a cold stimulus for initiation of flowering, whereas annuals lack any vernalization requirement. Cultivation of annuals is considered advantageous due to better integration into crop rotation and reduced production costs. However, available annual varieties lack a sufficient yield potential and essential oil content. Valuable genetic diversity exists within the biennial genepool, which can be transferred to the annual genepool by crossbreeding. According to literature, F1 plants from crosses between annual and biennial flowering types uniformly show annual flowering. Resulting F2 populations show a Mendelian 3:1 ratio between annual and biennial flowering types. This indicates a monogenic inheritance of vernalization requirement in caraway with dominant annual flowering. After crossbreeding of annual and biennial flowering types, the recessive allele for biennial flowering can remain within the breeding material over multiple generations. Thus, a genetic marker associated with the vernalization locus would be beneficial to detect the recessive allele in one selection step. To identify markers associated with vernalization requirement, we used genotyping by sequencing (GBS) data of 70 biennial and 67 annual flowering types. We conducted case-control studies and population differentiation-based tests. In total, 60 common single nucleotide polymorphism (SNP) markers were found that were significantly associated with vernalization requirement. Out of these, we developed 19 diagnostic markers. In future, these diagnostic markers might be tested in segregating populations. ; Kümmel (Carum carvi) kann in zweijährige und einjährige Blühtypen unterteilt werden. Zweijährige benötigen einen Kältereiz für die Blühinduktion, während Einjährige keinen Vernalisationsbedarf haben. Aufgrund der besseren Integration in die Fruchtfolge und der geringeren Produktionskosten wird der Anbau Einjähriger als vorteilhaft angesehen. Die ...
On the search for the vernalization locus in caraway (Carum carvi) using genotyping by sequencing data ; Auf der Suche nach dem Vernalisations-Locus in Kümmel (Carum carvi) mittels Genotyping by Sequencing
von Maydell, Daniel (author) / Keilwagen, Jens (author) / Lehnert, Heike (author) / Berner, Thomas (author) / Marthe, Frank (author)
2022-01-01
Article (Journal)
Electronic Resource
English , German
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