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Characterization of the meningeal immune compartment in ischemic stroke employing a single-cell atlas and flow cytometry of dural immune cells
The dura functions as a crucial immune niche located adjacent to the brain. However, the impact of pathological changes in the brain parenchyma on the dural immune compartment remains uncertain. This study aims to investigate the alterations in the compositions of dural immune cells under various pathological conditions and during the aging process. Besides, this study seeks to explore the roles of dural γδ T cells in the context of acute ischemic stroke (AIS). This study systematically searched and integrated dural single-cell RNA sequencing datasets from 10 published studies up to May 15, 2023, and established a single-cell atlas containing 120,893 cells. The single-cell atlas revealed notable heterogeneities in the percentages of various immune cells across six conditions, including baseline, aged, experimental autoimmune encephalomyelitis, traumatic brain injury, photothrombotic injury, and temporary middle cerebral artery occlusion. Furthermore, this study incorporated and analyzed dural single-cell RNA sequencing data at baseline, 1 day, and 3 days following AIS onset. The analysis showed an initial increasing followed by a subsequent decreasing trend in the proportion of dural γδ T cells post-AIS onset, which was confirmed by flow cytometric data. Enrichment analyses unveiled that the migratory capabilities and activation status of dural γδ T cells significantly increased as AIS progression. Additionally, this study conducted γδ T cell depletion prior to AIS modeling. The analysis of the frequency of dural immune cells in depleted mice showed that the impact of γδ T cells on the frequency of dural immune cells at day 3 post-AIS was minor, as the number of various dural immune cells were comparable, except for CD4+ T cells. Taken together, this study provides valuable insights into the disease-specific dural immune landscape. This study also postulates potential migration and activation patterns of dural γδ T cells following the onset of AIS. The observed increased migration abilities and heightened ...
Characterization of the meningeal immune compartment in ischemic stroke employing a single-cell atlas and flow cytometry of dural immune cells
The dura functions as a crucial immune niche located adjacent to the brain. However, the impact of pathological changes in the brain parenchyma on the dural immune compartment remains uncertain. This study aims to investigate the alterations in the compositions of dural immune cells under various pathological conditions and during the aging process. Besides, this study seeks to explore the roles of dural γδ T cells in the context of acute ischemic stroke (AIS). This study systematically searched and integrated dural single-cell RNA sequencing datasets from 10 published studies up to May 15, 2023, and established a single-cell atlas containing 120,893 cells. The single-cell atlas revealed notable heterogeneities in the percentages of various immune cells across six conditions, including baseline, aged, experimental autoimmune encephalomyelitis, traumatic brain injury, photothrombotic injury, and temporary middle cerebral artery occlusion. Furthermore, this study incorporated and analyzed dural single-cell RNA sequencing data at baseline, 1 day, and 3 days following AIS onset. The analysis showed an initial increasing followed by a subsequent decreasing trend in the proportion of dural γδ T cells post-AIS onset, which was confirmed by flow cytometric data. Enrichment analyses unveiled that the migratory capabilities and activation status of dural γδ T cells significantly increased as AIS progression. Additionally, this study conducted γδ T cell depletion prior to AIS modeling. The analysis of the frequency of dural immune cells in depleted mice showed that the impact of γδ T cells on the frequency of dural immune cells at day 3 post-AIS was minor, as the number of various dural immune cells were comparable, except for CD4+ T cells. Taken together, this study provides valuable insights into the disease-specific dural immune landscape. This study also postulates potential migration and activation patterns of dural γδ T cells following the onset of AIS. The observed increased migration abilities and heightened ...
Characterization of the meningeal immune compartment in ischemic stroke employing a single-cell atlas and flow cytometry of dural immune cells
Zha, Mingming (author) / Gelderblom, Mathias
2023-01-01
Theses
Electronic Resource
English
HMGB1 as a Key Mediator of Immune Mechanisms in Ischemic Stroke
British Library Online Contents | 2016
| Springer Verlag | 2025