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Characterization of extra-column peak broadening in capillary high-performance liquid chromatography
Ensuring reasonable energy prices, establishing reliable supply chains, ensuring the availability of resources and, last but not least, taking environmental protection into account are global challenges of the future. Corresponding challenges also play an increasingly central role in everyday analytical laboratory work. The search for countermeasures to these challenges is also becoming increasingly prevalent in the analytical laboratory. Miniaturization as one of the cornerstones of Green Analytical Chemistry (GAC) can help save resources in the form of solvents, energy and laboratory space, especially in the field of capillary liquid chromatography (cLC), without reducing the integrity of analytical data and even increasing sample throughput in the process. Despite advances in cLC development and research in recent years, and the disruptive nature of the technology, market penetration has still not been achieved. This is partly because there is a persistent assumption that the systems are unsuitable for routine operation due to a lack of robustness. In addition, although the number of commercial systems is increasing, current research is often not consistently implemented for example by sticking to the modular concept. This creates unnecessary capillary paths, increases the extra-column volume, which results in peak band broadening and thus poorer chromatographic efficiency. Therefore, the aim of this work is to investigate the influence of different factors on the extra-column band broadening in cLC, to lower barriers for the implementation of cLC and to develop a new system. The first part of the PhD thesis was mainly devoted to quantifying the influence of different extra-column volumes on peak band broadening and served to answer open questions in the current literature. To start, the influence of injection volume and pre-column volume was investigated for both isocratic and gradient elution as a function of retention factor. Subsequently, the focus was shifted to the influence of the post-column volume. ...
Characterization of extra-column peak broadening in capillary high-performance liquid chromatography
Ensuring reasonable energy prices, establishing reliable supply chains, ensuring the availability of resources and, last but not least, taking environmental protection into account are global challenges of the future. Corresponding challenges also play an increasingly central role in everyday analytical laboratory work. The search for countermeasures to these challenges is also becoming increasingly prevalent in the analytical laboratory. Miniaturization as one of the cornerstones of Green Analytical Chemistry (GAC) can help save resources in the form of solvents, energy and laboratory space, especially in the field of capillary liquid chromatography (cLC), without reducing the integrity of analytical data and even increasing sample throughput in the process. Despite advances in cLC development and research in recent years, and the disruptive nature of the technology, market penetration has still not been achieved. This is partly because there is a persistent assumption that the systems are unsuitable for routine operation due to a lack of robustness. In addition, although the number of commercial systems is increasing, current research is often not consistently implemented for example by sticking to the modular concept. This creates unnecessary capillary paths, increases the extra-column volume, which results in peak band broadening and thus poorer chromatographic efficiency. Therefore, the aim of this work is to investigate the influence of different factors on the extra-column band broadening in cLC, to lower barriers for the implementation of cLC and to develop a new system. The first part of the PhD thesis was mainly devoted to quantifying the influence of different extra-column volumes on peak band broadening and served to answer open questions in the current literature. To start, the influence of injection volume and pre-column volume was investigated for both isocratic and gradient elution as a function of retention factor. Subsequently, the focus was shifted to the influence of the post-column volume. ...
Characterization of extra-column peak broadening in capillary high-performance liquid chromatography
Werres, Tobias (author) / Schmidt, Torsten Claus
2023-05-03
Theses
Electronic Resource
English
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