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Ultrasound as a apid and Low-cost extraction procedure to obtain Anthocyanin-based colorants from Prunus spinosa L. Fruit epicarp: comparative study with conventional heat-based extraction
An ultrasound rapid and low-cost procedure for anthocyanin-based colorants from Prunus spinosa L. fruit epicarp was developed, and the advantages were compared with conventional heat-based extraction. To obtain the conditions that maximize anthocyanins’ extraction, a response surface methodology was applied using the variables of time, temperature, and ethanol content, in the case of heat extraction, whereas for ultrasound assisted extraction, temperature was replaced by ultrasound power. Two anthocyanin compounds were identified by HPLC-DAD-ESI/MS—namely, cyanidin 3-rutinoside and peonidin 3-rutinoside. The responses used were the extraction yield and the content of the identified anthocyanins. Ultrasound extraction was the most effective method at 5.00 0.15 min, 400.00 32.00 W, and 47.98% 2.88% of ethanol obtaining 68.60% 2.06% of extracted residue, with an anthocyanin content of 18.17 mg/g (extract-basis) and 11.76 mg/g (epicarp-basis). Overall, a viable green process was achieved that could be used to support pilot-scale studies for industrial production of anthocyanin-based colorants from P. spinosa fruit epicarp. ; The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2013), L. Barros and C. Pereira research contract; to FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E.; to European Regional Development Fund (ERDF) through the Regional Operational Program North 2020, within the scope of Project NORTE-01-0145-FEDER-023289: DeCodE and project Mobilizador Norte-01-0247-FEDER-024479: ValorNatural®. This work was also financially supported by the following: Project POCI-01-0145-FEDER-006984, Associate Laboratory LSRE-LCM funded by FEDER through COMPETE2020, Programa Operacional Competitividade e Internacionalização (POCI), and national funds through FCT. The authors thank the GAIN (Xunta de Galicia) for financial support (P.P. 0000 421S 140.08) to ...
Ultrasound as a apid and Low-cost extraction procedure to obtain Anthocyanin-based colorants from Prunus spinosa L. Fruit epicarp: comparative study with conventional heat-based extraction
An ultrasound rapid and low-cost procedure for anthocyanin-based colorants from Prunus spinosa L. fruit epicarp was developed, and the advantages were compared with conventional heat-based extraction. To obtain the conditions that maximize anthocyanins’ extraction, a response surface methodology was applied using the variables of time, temperature, and ethanol content, in the case of heat extraction, whereas for ultrasound assisted extraction, temperature was replaced by ultrasound power. Two anthocyanin compounds were identified by HPLC-DAD-ESI/MS—namely, cyanidin 3-rutinoside and peonidin 3-rutinoside. The responses used were the extraction yield and the content of the identified anthocyanins. Ultrasound extraction was the most effective method at 5.00 0.15 min, 400.00 32.00 W, and 47.98% 2.88% of ethanol obtaining 68.60% 2.06% of extracted residue, with an anthocyanin content of 18.17 mg/g (extract-basis) and 11.76 mg/g (epicarp-basis). Overall, a viable green process was achieved that could be used to support pilot-scale studies for industrial production of anthocyanin-based colorants from P. spinosa fruit epicarp. ; The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2013), L. Barros and C. Pereira research contract; to FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E.; to European Regional Development Fund (ERDF) through the Regional Operational Program North 2020, within the scope of Project NORTE-01-0145-FEDER-023289: DeCodE and project Mobilizador Norte-01-0247-FEDER-024479: ValorNatural®. This work was also financially supported by the following: Project POCI-01-0145-FEDER-006984, Associate Laboratory LSRE-LCM funded by FEDER through COMPETE2020, Programa Operacional Competitividade e Internacionalização (POCI), and national funds through FCT. The authors thank the GAIN (Xunta de Galicia) for financial support (P.P. 0000 421S 140.08) to ...
Ultrasound as a apid and Low-cost extraction procedure to obtain Anthocyanin-based colorants from Prunus spinosa L. Fruit epicarp: comparative study with conventional heat-based extraction
Leichtweis, Maria Gabriela (author) / Pereira, Carla (author) / Prieto Lage, Miguel A. (author) / Barreiro, M.F. (author) / Baraldi, Ilton J. (author) / Barros, Lillian (author) / Ferreira, Isabel C.F.R. (author)
2019-01-01
doi:10.3390/molecules24030573
Article (Journal)
Electronic Resource
English
DDC:
690
Architectural publications index on disc : APId
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