A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Expressionsanalyse der Prostaglandin-E-Synthase des Adenokarzinoms des distalen Ösophagus auf mRNA-Ebene ; mRNA-Expressionanaysis of the Prostaglandin-E-Synthasis of the Adenocarcinoma of the distal oesophagus
Die Inzidenz des Adenokarzinoms des distalen Ösophagus steigt in der westlichen Welt rapide an - die Prognose bleibt schlecht. Durch chronischen Reflux kommt es zu metaplastischem Umbau von Speiseröhrengewebe. Die Beteiligung von Prostaglandin E2 (PGE2) an der Karzinogenese wird postuliert. PGE2 soll insb. bei tumorbedingter Neovaskularisation und Tumorwachstum Mediatorfunktion haben. PGE2 entsteht durch Prostaglandin-E-Synthasen (PGES). Ziel der Arbeit war eine Expressionsanalyse der PGES. An 123 Tumorproben führten wir eine semiquantitative Genanalyse (PGES/GAPDH ratio) auf mRNA-Ebene durch. Es zeigten sich statistisch-hochsignifikante Korrelationen von PGES zu COX-1 und -2, VEGF-A und -C, sowie TGFbeta und Mikrogefäßdichte. Keine Korrelation bestehen zu den untersuchten klin.-path. Parametern. Allerdings hat der Mittelwert der PGES prognostische Relevanz. Der Zusammenhang mit Angiogenese und Zellregulation könnte zukünftig therapeutische und präventive Funktion besitzen. ; The incidence of the adenocarcinoma of the distal oesophagus is increasing - majorly among white males in the western world. Its prognosis is bad. Chronic gastroduodenal reflux (GERD) causes metaplasia of oesophagus tissue to Barrett esophagus - and cancer development in later progress. Prostaglangin E2 (PGE2) has several functions. Some involvement in carcinogenesis has been discussed recently. PGE2 is a mediator, especially for neovascularisation in tumour development. PGE2 is synthesised by Prostaglandin-E-Synthasis (PGES). The main aspect of this study was an expression analysis of PGES. We did semiquantitative genetic analysis of 123 paraffin-blocks of adenocarcinoma on mRNA-level (TaqMan). The mean mRNA-Quantity (PGES/GAPDH ratio) was 10.72 [range 0.35-68.41]. Significant correlations of PGES with COX-1 and -2, VEGF-A and -C, and TGFbeta (p<0.001) were found. The correlation with the microvessel density (MCD) was shown, too. The amount of PGES did not correlate significantly with any clinical-pathological parameter: UICC classification (T: p=0.191; N: p=0.905), WHO classification (p=0.651), R classification (p=0.655), grading G (p=0.464), lymphatic invadion L (p=0.541). The amount of PGES (PGES level above or below the median, Kaplan-Meier) is significantly correlated with survival. Low PGES is associated with prolonged survival. According to this study, PGES of the distal oesophagus is associated with factors of angiogenesis and cellular regulation.
Expressionsanalyse der Prostaglandin-E-Synthase des Adenokarzinoms des distalen Ösophagus auf mRNA-Ebene ; mRNA-Expressionanaysis of the Prostaglandin-E-Synthasis of the Adenocarcinoma of the distal oesophagus
Die Inzidenz des Adenokarzinoms des distalen Ösophagus steigt in der westlichen Welt rapide an - die Prognose bleibt schlecht. Durch chronischen Reflux kommt es zu metaplastischem Umbau von Speiseröhrengewebe. Die Beteiligung von Prostaglandin E2 (PGE2) an der Karzinogenese wird postuliert. PGE2 soll insb. bei tumorbedingter Neovaskularisation und Tumorwachstum Mediatorfunktion haben. PGE2 entsteht durch Prostaglandin-E-Synthasen (PGES). Ziel der Arbeit war eine Expressionsanalyse der PGES. An 123 Tumorproben führten wir eine semiquantitative Genanalyse (PGES/GAPDH ratio) auf mRNA-Ebene durch. Es zeigten sich statistisch-hochsignifikante Korrelationen von PGES zu COX-1 und -2, VEGF-A und -C, sowie TGFbeta und Mikrogefäßdichte. Keine Korrelation bestehen zu den untersuchten klin.-path. Parametern. Allerdings hat der Mittelwert der PGES prognostische Relevanz. Der Zusammenhang mit Angiogenese und Zellregulation könnte zukünftig therapeutische und präventive Funktion besitzen. ; The incidence of the adenocarcinoma of the distal oesophagus is increasing - majorly among white males in the western world. Its prognosis is bad. Chronic gastroduodenal reflux (GERD) causes metaplasia of oesophagus tissue to Barrett esophagus - and cancer development in later progress. Prostaglangin E2 (PGE2) has several functions. Some involvement in carcinogenesis has been discussed recently. PGE2 is a mediator, especially for neovascularisation in tumour development. PGE2 is synthesised by Prostaglandin-E-Synthasis (PGES). The main aspect of this study was an expression analysis of PGES. We did semiquantitative genetic analysis of 123 paraffin-blocks of adenocarcinoma on mRNA-level (TaqMan). The mean mRNA-Quantity (PGES/GAPDH ratio) was 10.72 [range 0.35-68.41]. Significant correlations of PGES with COX-1 and -2, VEGF-A and -C, and TGFbeta (p<0.001) were found. The correlation with the microvessel density (MCD) was shown, too. The amount of PGES did not correlate significantly with any clinical-pathological parameter: UICC classification (T: p=0.191; N: p=0.905), WHO classification (p=0.651), R classification (p=0.655), grading G (p=0.464), lymphatic invadion L (p=0.541). The amount of PGES (PGES level above or below the median, Kaplan-Meier) is significantly correlated with survival. Low PGES is associated with prolonged survival. According to this study, PGES of the distal oesophagus is associated with factors of angiogenesis and cellular regulation.
Expressionsanalyse der Prostaglandin-E-Synthase des Adenokarzinoms des distalen Ösophagus auf mRNA-Ebene ; mRNA-Expressionanaysis of the Prostaglandin-E-Synthasis of the Adenocarcinoma of the distal oesophagus
2008-06-11
Theses
Electronic Resource
German
survival , PCR , Angiogenese , PGES , COX , Prostaglandin-E-Synthasis , cancer , Speiseröhre , adenocarcinoma , Überleben , cellular regulation , GERD , TaqMan , Medizin und Gesundheit , Krebs , Barrettkarzinom , Zellregulation , oesophagus , angiogenesis , microvessel density , genetic analyis , Adenokarzinom , Prostaglandin-E-Synthase , barrett carcinoma , Mikrogefäßdichte , ddc:610 , Genanalyse
Synthesis of (±)-15-deoxy-Δ12,14-prostaglandin J2 and Δ12-prostaglandin J2 15-acetate methyl esters
| British Library Online Contents | 2015
| British Library Online Contents | 1998
Benzo(a)pyrene induces p73 mRNA expression and necrosis in human lung adenocarcinoma H1299 cells
| Online Contents | 2012
Cyclopentenone blocks for 15-deoxy-Δ 12,14 -prostaglandin J2
| British Library Online Contents | 2011