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The effect of organic solvents on biofilm formation of the thermoacidophilic Archaeon Sulfolobus acidocaldarius ; Der Effekt von organischen Lösemitteln auf die Biofilmbildung des thermoazidophilen Archaeon Sulfolobus acidocaldarius
Sulfolobus acidocaldarius is a thermoacidophilic Crenarchaeon (78°C and pH 2-3) with established genetic systems. With these properties, S. acidocaldarius offers high potential for biotechnological applications as alternative host for the production of bulk and fine chemicals, solvents and second generation biofuels, which are, however, often toxic to cells. Biofilms, the dominant lifestyle of microorganisms, were shown to confer higher resistance towards solvent exposure and hence turn more and more into research focus for biotechnological applications. Although S. acidocaldarius is known to form biofilms, the development of suitable incubation systems is still in its beginning and also the influence of organic solvents on S. acidocaldarius has only rarely been studied. In this work, incubation systems for the cultivation of submerse biofilms of S. acidocaldarius cells on glass and polystyrene surfaces in microtiter plates, μ- and Petri-dishes as well as a flow-through system were successfully developed and optimized. These newly developed methods enabled detailed microscopic analyses and also yielded sufficient amounts of biofilms for isolation and analyses of extracellular polymeric substances (EPS) as well as for application of various -omics methods. The analyses were performed in presence and absence of organic solvents (with special respect to 1-butanol) and the results were compared to shaking cultures. S. acidocaldarius formed biofilms even in shaking cultures in Erlenmeyer flasks at the liquid/glass/air interface as a (stress)response to sublethal concentration of different solvents. In accordance with shaking cultures, in a 96-well plate static incubation system, the tendency of enhanced biofilm formation as a response to sublethal concentrations of 1-butanol (<1.5% (v/v)) was visible. By the use of an adapted resazurin assay, indicating respiratory activity, the viability of biofilm cells was confirmed colorimetrically. Furthermore, in static incubation systems e.g. on glass slides, S. ...
The effect of organic solvents on biofilm formation of the thermoacidophilic Archaeon Sulfolobus acidocaldarius ; Der Effekt von organischen Lösemitteln auf die Biofilmbildung des thermoazidophilen Archaeon Sulfolobus acidocaldarius
Sulfolobus acidocaldarius is a thermoacidophilic Crenarchaeon (78°C and pH 2-3) with established genetic systems. With these properties, S. acidocaldarius offers high potential for biotechnological applications as alternative host for the production of bulk and fine chemicals, solvents and second generation biofuels, which are, however, often toxic to cells. Biofilms, the dominant lifestyle of microorganisms, were shown to confer higher resistance towards solvent exposure and hence turn more and more into research focus for biotechnological applications. Although S. acidocaldarius is known to form biofilms, the development of suitable incubation systems is still in its beginning and also the influence of organic solvents on S. acidocaldarius has only rarely been studied. In this work, incubation systems for the cultivation of submerse biofilms of S. acidocaldarius cells on glass and polystyrene surfaces in microtiter plates, μ- and Petri-dishes as well as a flow-through system were successfully developed and optimized. These newly developed methods enabled detailed microscopic analyses and also yielded sufficient amounts of biofilms for isolation and analyses of extracellular polymeric substances (EPS) as well as for application of various -omics methods. The analyses were performed in presence and absence of organic solvents (with special respect to 1-butanol) and the results were compared to shaking cultures. S. acidocaldarius formed biofilms even in shaking cultures in Erlenmeyer flasks at the liquid/glass/air interface as a (stress)response to sublethal concentration of different solvents. In accordance with shaking cultures, in a 96-well plate static incubation system, the tendency of enhanced biofilm formation as a response to sublethal concentrations of 1-butanol (<1.5% (v/v)) was visible. By the use of an adapted resazurin assay, indicating respiratory activity, the viability of biofilm cells was confirmed colorimetrically. Furthermore, in static incubation systems e.g. on glass slides, S. ...
The effect of organic solvents on biofilm formation of the thermoacidophilic Archaeon Sulfolobus acidocaldarius ; Der Effekt von organischen Lösemitteln auf die Biofilmbildung des thermoazidophilen Archaeon Sulfolobus acidocaldarius
Benninghoff, Jens Carlo (author) / Siebers, Bettina
2020-10-12
Theses
Electronic Resource
English
Polyphosphate metabolism and profiling of myristoylation in Sulfolobus acidocaldarius
| BASE | 2023