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Microbial ecology of industrial activated sludge process: linking functional diversity to system performance ; Mikrobielle Ökologie industrieller Belebtschlammverfahren: Verknüpfung von Funktionsvielfalt mit Systemleistung
Understanding the microbial ecology of industrial activated sludge requires the linking of observed population dynamics to specific functions, such as degradation processes (nutrient removal) or disturbance events (bulking and foaming). Since functional diversity is generally assumed to be positively correlated to system stability, it is important to assess how diversity levels influence process performance. The present study underlines the value of recognizing and attributing functions to activated sludge microbes in industrial environments for enhanced process monitoring and control. Two case studies featuring full-scale industrial activated sludge plants were conducted using advanced molecular tools, e.g. Illumina sequencing, real-time PCR and FISH. Sequencing results showed that the distribution of high bacteria taxa was highly represented by Actinobacteria, Bacteroidetes, Proteobacteria and unclassified Bacteria in most of the samples, while at the species level, each biological step had a unique bacterial composition. Multivariate analysis of the sequencing data revealed that the most influencing variables driving community composition were temperature, pH, dissolved oxygen, sludge age and fatty acids. Core bacteria species were identified and classified according to their function in the system, i.e. Aequorovita, Flavobacterium, Bacterium Kaz2 and M. parvicella. Using newly designed 16S rRNA primers and probes, functional bacteria were quantified by real-time PCR and characterized by FISH. One of the most significant findings was the characterization of the Bacterium Kaz2, which is an unclassified bacterium. According to FISH results, it was determined that this bacterium belongs to the phylum Bacteroidetes. Regarding its morphological characteristics, FISH also showed that the Bacterium Kaz2 is a large coccus that grows in irregular clusters in a size range of 5 to 25 µm inside the sludge flocs. Its growth is promoted by high temperatures and low values of sludge age and it can easily adapt to aerobic ...
Microbial ecology of industrial activated sludge process: linking functional diversity to system performance ; Mikrobielle Ökologie industrieller Belebtschlammverfahren: Verknüpfung von Funktionsvielfalt mit Systemleistung
Understanding the microbial ecology of industrial activated sludge requires the linking of observed population dynamics to specific functions, such as degradation processes (nutrient removal) or disturbance events (bulking and foaming). Since functional diversity is generally assumed to be positively correlated to system stability, it is important to assess how diversity levels influence process performance. The present study underlines the value of recognizing and attributing functions to activated sludge microbes in industrial environments for enhanced process monitoring and control. Two case studies featuring full-scale industrial activated sludge plants were conducted using advanced molecular tools, e.g. Illumina sequencing, real-time PCR and FISH. Sequencing results showed that the distribution of high bacteria taxa was highly represented by Actinobacteria, Bacteroidetes, Proteobacteria and unclassified Bacteria in most of the samples, while at the species level, each biological step had a unique bacterial composition. Multivariate analysis of the sequencing data revealed that the most influencing variables driving community composition were temperature, pH, dissolved oxygen, sludge age and fatty acids. Core bacteria species were identified and classified according to their function in the system, i.e. Aequorovita, Flavobacterium, Bacterium Kaz2 and M. parvicella. Using newly designed 16S rRNA primers and probes, functional bacteria were quantified by real-time PCR and characterized by FISH. One of the most significant findings was the characterization of the Bacterium Kaz2, which is an unclassified bacterium. According to FISH results, it was determined that this bacterium belongs to the phylum Bacteroidetes. Regarding its morphological characteristics, FISH also showed that the Bacterium Kaz2 is a large coccus that grows in irregular clusters in a size range of 5 to 25 µm inside the sludge flocs. Its growth is promoted by high temperatures and low values of sludge age and it can easily adapt to aerobic ...
Microbial ecology of industrial activated sludge process: linking functional diversity to system performance ; Mikrobielle Ökologie industrieller Belebtschlammverfahren: Verknüpfung von Funktionsvielfalt mit Systemleistung
Gallegos, Erika Lizette De Leon (author) / Denecke, Martin
2018-07-24
Theses
Electronic Resource
English
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