A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Development of a Rapid Loop-Mediated Isothermal Amplification Assay for the Detection of Dothistroma septosporum
A Loop-Mediated Isothermal Amplification (LAMP) assay was developed for the detection of the pine pathogen Dothistroma septosporum (G. Dorog.) M. Morelet. The specificity of the LAMP assay was tested using a selection of pine needle fungi, including Dothistroma pini Hulbary, and Lecanosticta acicola (Thüm.) Syd.; only D. septosporum DNA was amplified by the test. In terms of sensitivity, the assay was able to detect as little as 1 pg of total D. septosporum DNA. This assay enables DNA extracted from diseased host needles to be rapidly tested for the presence of D. septosporum using relatively simple to operate equipment away from a fully equipped molecular biology laboratory.
Development of a Rapid Loop-Mediated Isothermal Amplification Assay for the Detection of Dothistroma septosporum
A Loop-Mediated Isothermal Amplification (LAMP) assay was developed for the detection of the pine pathogen Dothistroma septosporum (G. Dorog.) M. Morelet. The specificity of the LAMP assay was tested using a selection of pine needle fungi, including Dothistroma pini Hulbary, and Lecanosticta acicola (Thüm.) Syd.; only D. septosporum DNA was amplified by the test. In terms of sensitivity, the assay was able to detect as little as 1 pg of total D. septosporum DNA. This assay enables DNA extracted from diseased host needles to be rapidly tested for the presence of D. septosporum using relatively simple to operate equipment away from a fully equipped molecular biology laboratory.
Development of a Rapid Loop-Mediated Isothermal Amplification Assay for the Detection of Dothistroma septosporum
Colin L. Myrholm (author) / Bradley D. Tomm (author) / Renate Heinzelmann (author) / Nicolas Feau (author) / Richard C. Hamelin (author) / Rebecca McDougal (author) / Richard C. Winkworth (author) / Tod D. Ramsfield (author)
2021
Article (Journal)
Electronic Resource
Unknown
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| British Library Conference Proceedings | 2013
| Wiley | 2022