A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Male-Specific Sequence in Populus simonii Provides Insights into Gender Determination of Poplar
The genus Populus is composed of dioecious woody plants and adult females produce large numbers of seed hairs that can affect public health and pose a potential fire risk. However, it is difficult to distinguish between males and females based on their morphology at the seedling stage. Therefore, developing a technology that identifies the gender of poplar seedlings is crucial for controlling seed hairs. In this study, we developed an approach for the early gender identification of Tacamahaca and Aigeiros species based on the male-specific sequence in Populus simonii. The gender of Tacamahaca and Aigeiros species can be accurately identified by PCR. The sequencing results showed that the male-specific sequence was conserved in P. simonii and its F1 progenies. Interestingly, there were three nucleobase differences between Tacamahaca and Aigeiros species. Sequence alignment showed that the male-specific sequence had not been assembled on the pseudochromosome. Subsequently, fluorescence in situ hybridization (FISH) was used to locate this specific sequence at the short arm end of chromosome 19 in male P. simonii. This study provides an efficient and convenient method for early gender determination of Tacamahaca and Aigeiros species and lays the groundwork for exploring key sex-determination genes.
Male-Specific Sequence in Populus simonii Provides Insights into Gender Determination of Poplar
The genus Populus is composed of dioecious woody plants and adult females produce large numbers of seed hairs that can affect public health and pose a potential fire risk. However, it is difficult to distinguish between males and females based on their morphology at the seedling stage. Therefore, developing a technology that identifies the gender of poplar seedlings is crucial for controlling seed hairs. In this study, we developed an approach for the early gender identification of Tacamahaca and Aigeiros species based on the male-specific sequence in Populus simonii. The gender of Tacamahaca and Aigeiros species can be accurately identified by PCR. The sequencing results showed that the male-specific sequence was conserved in P. simonii and its F1 progenies. Interestingly, there were three nucleobase differences between Tacamahaca and Aigeiros species. Sequence alignment showed that the male-specific sequence had not been assembled on the pseudochromosome. Subsequently, fluorescence in situ hybridization (FISH) was used to locate this specific sequence at the short arm end of chromosome 19 in male P. simonii. This study provides an efficient and convenient method for early gender determination of Tacamahaca and Aigeiros species and lays the groundwork for exploring key sex-determination genes.
Male-Specific Sequence in Populus simonii Provides Insights into Gender Determination of Poplar
Ziyue Wang (author) / Yijing Lei (author) / Guanqing Liu (author) / Yihang Ning (author) / Runxin Ni (author) / Tao Zhang (author) / Mengli Xi (author)
2023
Article (Journal)
Electronic Resource
Unknown
Metadata by DOAJ is licensed under CC BY-SA 1.0
Populus simonii Carr. Reduces Wind Erosion and Improves Soil Properties in Northern China
| DOAJ | 2019
Damage Characteristics of Populus Simonii Root Systems at Different Cracks in Mining Subsidence Area
| DOAJ | 2022
Effects of Zn Exposure on Populus simonii Seedling Growth and Its Resistance to Leaf Rust
| DOAJ | 2023