A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Influence of Light Intensity and Photoperiod on the Photoautotrophic Growth and Lipid Content of the Microalgae Verrucodesmus verrucosus in a Photobioreactor
Microalgal biomass has the capacity to accumulate relatively large quantities of triacylglycerides (TAG) for the conversion of methyl esters of fatty acids (FAME) which has made microalgae a desirable alternative for the production of biofuels. In the present work Verrucodesmus verrucosus was evaluated under autotrophic growth conditions as a suitable source of oil for biodiesel production. For this purpose BG11 media were evaluated in three different light:dark photoperiods (L:D; 16:08; 12:12; 24:0) and light intensities (1000, 2000 and 3000 Lux) in a photobioreactor with a capacity of three liters; the evaluation of the microalgal biomass was carried out through the cell count with the use of the Neubauer chamber followed by the evaluation of the kinetic growth parameters. So, the lipid accumulation was determined through the lipid extraction with a Soxhlet system. Finally, the fatty acid profile of the total pooled lipids was determined using gas chromatography-mass spectroscopy (GC-MS). The results demonstrate that the best conditions are a photoperiod of 12 light hours and 12 dark hours with BG11 medium in a 3 L tubular photobioreactor with 0.3% CO2, 25 °C and 2000 Lux, allowing a lipid accumulation of 50.42%. Palmitic acid is identified as the most abundant fatty acid at 44.90%.
Influence of Light Intensity and Photoperiod on the Photoautotrophic Growth and Lipid Content of the Microalgae Verrucodesmus verrucosus in a Photobioreactor
Microalgal biomass has the capacity to accumulate relatively large quantities of triacylglycerides (TAG) for the conversion of methyl esters of fatty acids (FAME) which has made microalgae a desirable alternative for the production of biofuels. In the present work Verrucodesmus verrucosus was evaluated under autotrophic growth conditions as a suitable source of oil for biodiesel production. For this purpose BG11 media were evaluated in three different light:dark photoperiods (L:D; 16:08; 12:12; 24:0) and light intensities (1000, 2000 and 3000 Lux) in a photobioreactor with a capacity of three liters; the evaluation of the microalgal biomass was carried out through the cell count with the use of the Neubauer chamber followed by the evaluation of the kinetic growth parameters. So, the lipid accumulation was determined through the lipid extraction with a Soxhlet system. Finally, the fatty acid profile of the total pooled lipids was determined using gas chromatography-mass spectroscopy (GC-MS). The results demonstrate that the best conditions are a photoperiod of 12 light hours and 12 dark hours with BG11 medium in a 3 L tubular photobioreactor with 0.3% CO2, 25 °C and 2000 Lux, allowing a lipid accumulation of 50.42%. Palmitic acid is identified as the most abundant fatty acid at 44.90%.
Influence of Light Intensity and Photoperiod on the Photoautotrophic Growth and Lipid Content of the Microalgae Verrucodesmus verrucosus in a Photobioreactor
Laura Vélez-Landa (author) / Héctor Ricardo Hernández-De León (author) / Yolanda Del Carmen Pérez-Luna (author) / Sabino Velázquez-Trujillo (author) / Joel Moreira-Acosta (author) / Roberto Berrones-Hernández (author) / Yazmin Sánchez-Roque (author)
2021
Article (Journal)
Electronic Resource
Unknown
Metadata by DOAJ is licensed under CC BY-SA 1.0
Exploration of Microalgae Photobioreactor (PBR) in Tropical Climate Building Envelope
| BASE | 2020
A novel sequence batch membrane carbonation photobioreactor developed for microalgae cultivation
| Springer Verlag | 2019
| European Patent Office | 2020
CFD modeling of CO2 fixation by microalgae cultivated in a lab scale photobioreactor
| BASE | 2025