A platform for research: civil engineering, architecture and urbanism
A platform for research: civil engineering, architecture and urbanism
Epigenetic Integrity of Orthodox Seeds Stored under Conventional and Cryogenic Conditions
The level of 5-methylcytosine (m5C) in DNA has been observed to change in plants in response to biotic and abiotic stress factors. Little information has been reported on alterations in DNA methylation in orthodox tree seeds in response to storage conditions. In the current study, epigenetic integrity was analyzed in seeds of Pyrus communis L. in response to conventional and cryogenic storage. The results indicate that conventional storage under optimal conditions resulted in a significant increase in m5C. In contrast, a decrease in m5C level after cryostorage at high water content (WC) was observed, not only in seeds but also in 3-month-old seedlings which were smaller than seedlings obtained from seeds cryostored at optimal WC. This shows that non-optimal cryostorage conditions increase epigenetic instability in seeds and seedlings. Optimal procedures for germplasm conservation are very important for germplasm banking since they have serious implications for the quality of stored collections. Maintaining epigenetic integrity during WC adjustment and optimal storage is a characteristic feature of orthodox seeds. The current results underline the importance of proper protocols and techniques for conventional storage and particularly cryopreservation as a method for conservation of true-to-type germplasm for long periods.
Epigenetic Integrity of Orthodox Seeds Stored under Conventional and Cryogenic Conditions
The level of 5-methylcytosine (m5C) in DNA has been observed to change in plants in response to biotic and abiotic stress factors. Little information has been reported on alterations in DNA methylation in orthodox tree seeds in response to storage conditions. In the current study, epigenetic integrity was analyzed in seeds of Pyrus communis L. in response to conventional and cryogenic storage. The results indicate that conventional storage under optimal conditions resulted in a significant increase in m5C. In contrast, a decrease in m5C level after cryostorage at high water content (WC) was observed, not only in seeds but also in 3-month-old seedlings which were smaller than seedlings obtained from seeds cryostored at optimal WC. This shows that non-optimal cryostorage conditions increase epigenetic instability in seeds and seedlings. Optimal procedures for germplasm conservation are very important for germplasm banking since they have serious implications for the quality of stored collections. Maintaining epigenetic integrity during WC adjustment and optimal storage is a characteristic feature of orthodox seeds. The current results underline the importance of proper protocols and techniques for conventional storage and particularly cryopreservation as a method for conservation of true-to-type germplasm for long periods.
Epigenetic Integrity of Orthodox Seeds Stored under Conventional and Cryogenic Conditions
Beata P. Plitta-Michalak (author) / Mirosława Z. Naskręt-Barciszewska (author) / Jan Barciszewski (author) / Paweł Chmielarz (author) / Marcin Michalak (author)
2021
Article (Journal)
Electronic Resource
Unknown
Metadata by DOAJ is licensed under CC BY-SA 1.0
| TIBKAT | 2020
Accelerometer Spiking Under Cryogenic Conditions
| British Library Online Contents | 2001
Greek orthodox churches in Anatolia
| British Library Conference Proceedings | 1997
The Generalisation of Orthodox Crystallography
| British Library Online Contents | 1994
Integrity of repaired concrete under repeated loading conditions
| British Library Conference Proceedings | 1996